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Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice  Eisei Sohara, M.D., Ph.D., Otoya Ueda, M.Sc., Takanori Tachibe, A.S.,

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1 Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice 
Eisei Sohara, M.D., Ph.D., Otoya Ueda, M.Sc., Takanori Tachibe, A.S., Toshio Hani, M.Sc., Kou-ichi Jishage, Ph.D., Tatemitsu Rai, M.D., Ph.D., Sei Sasaki, M.D., Ph.D., Shinichi Uchida, M.D., Ph.D.  Fertility and Sterility  Volume 87, Issue 3, Pages (March 2007) DOI: /j.fertnstert Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

2 FIGURE 1 (A) RT-PCR of AQP7 using mRNA from testis of wild-type (+/+) and AQP7 knockout (−/−) mice. (B) Immunoblot of kidney homogenate probed with anti-AQP7 antibody. (C) Testicular histology of mice of the indicated genotype. (D) Number of offspring from wild-type female mice mated with wild-type male mice (+/+) and those from AQP7 knockout female mice mated with AQP7 knockout male mice (−/−). (E) Testicular weight–to–body weight ratio. (F) Ratio of epididymis weight to body weight. Sohara. Reproductive function in AQP7 -/- mice. Fertil Steril 2007. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

3 FIGURE 2 (A) Daily sperm production. (B) Percentage of motile sperm 1.5 h after spreading the sperm onto TYH medium. (C) Percentage of motile sperm 24 h after spreading the sperm onto TYH medium. Sohara. Reproductive function in AQP7 -/- mice. Fertil Steril 2007. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

4 FIGURE 3 Percentage of morphologically normal sperm.
Sohara. Reproductive function in AQP7 -/- mice. Fertil Steril 2007. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions

5 FIGURE 4 Morphometric analysis of the spermatozoa obtained from wild-type and AQP7 knockout mice. Each bar represents mean and standard deviation of the full length (A) and the area of the head (B) for the spermatozoa obtained from wild-type or AQP7 knockout mice. Sohara. Reproductive function in AQP7 -/- mice. Fertil Steril 2007. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2007 American Society for Reproductive Medicine Terms and Conditions


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