1. Correlation between ICAM-1 and functional recovery of piglet myocardium with leukocyte-depleted reperfusion 
Jeong Ryul Lee, MD, Jae Jin Han, MD, Jeong Wook Seo, MD 
The Annals of Thoracic Surgery 
Volume 70, Issue 5, Pages (November 2000)
DOI: /S (00)01938-X

2. Fig 1
Diagram of the isolated working heart perfusion circuit. Donor blood, either passing through a leukocyte filter (group 2) or not passing through (group 1), is pumped to either the aortic root or a reservoir that supplies the left atrium. The afterload is set to 60 mm Hg by the height of the aortic column. Cardiac output is measured by timed collections of the coronary sinus effluent and overflow from the aortic column. (LF = leukocyte filter; CO = cardiac output; CSE = coronary sinus effluent; HR = heated reservoir; Ao = aorta; PA = pulmonary artery; RA = right atrium; LA = left atrium; RV = right ventricle; LV = left ventricle.)
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)

3. Fig 2
Immunofluorescent micrograph of the myocardium using antibody against ICAM-1. A, Trace (+) immunoreaction (open arrowheads) of the venules and arterioles shows incomplete circles at the vascular endothelium. B, Moderate (+++) immunoreaction shows fluorescence (arrowheads) at the vascular endothelium.
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)

4. Fig 3 The average efficacy of leukocyte depletion was 96.0% ± 2.3%.
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)

5. Fig 4
The mean grades of tissue ICAM-1 expression in the myocardium, were significantly higher in the control group (group 1) than the leukocyte-depleted group (group 2) at 3 hours (p = 0.047) and 4 hours (p = ) after the onset of cardiac reperfusion.
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)

6. Fig 5
The mean stroke work indices were significantly greater in the leukocyte-depleted group (group 2) than in the control group (group 1) at 2, 3, and 4 hours after the onset of reperfusion (p = , p = , and p = , respectively).
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)

7. Fig 6
The myocardial water content, expressed as percent wet–dry ratio, was not significantly different between the two groups (LD = leukocyte-depleted group).
The Annals of Thoracic Surgery  , DOI: ( /S (00)01938-X)