1. Fig. 2 Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART results in successive increases in elongated, polyadenylated, and multiply spliced HIV transcripts.
Activation of freshly isolated CD4+ T cells from HIV-infected patients on ART results in successive increases in elongated, polyadenylated, and multiply spliced HIV transcripts. (A to J) Using RT-ddPCR, read-through (A and F), total (B and G), elongated (C and H), polyadenylated (D and I), and multiply spliced Tat-Rev (E and J) HIV transcripts were measured in unstimulated CD4+ T cells (day 0) and ex vivo activated CD4+ T cells (day 2) from 12 ART-suppressed individuals. (A to E) Effect of activation, as normalized by RNA mass. Measures of each HIV transcript were normalized to 1 μg of cellular RNA (~106 unstimulated cells) to show how activation changes amounts of each HIV transcript in relation to global cellular transcription. (F to J) Effect of activation, as normalized by DNA mass. HIV RNA measures in the unstimulated and activated cells were also normalized to 106 cells using the cell equivalents recovered in the extracted DNA (a surrogate for cell numbers in the activated cells; available for 11 of 12 participants). Each graph shows the change in HIV RNA from day 0 (unstimulated) to day 2 (activated) on a log scale; each color represents a separate individual; comparisons were performed using the Wilcoxon signed-rank test.
Steven A. Yukl et al., Sci Transl Med 2018;10:eaap9927
Published by AAAS