1. Volume 67, Issue 1, Pages 314-320 (January 2005)
Studies on unfolded β2-microglobulin at C-terminal in dialysis-related amyloidosis 
Yoshihiro Motomiya, Yukio Ando, Katsuki Haraoka, Xuguo Sun, Hiroyuki Morita, Izumi Amano, Tomonori Uchimura, Ikuro Maruyama, M.D. 
Kidney International 
Volume 67, Issue 1, Pages (January 2005)
DOI: /j x
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2. Figure 1 MALDI mass spectra of synthesized peptide.
Kidney International  , DOI: ( /j x)
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3. Figure 2
Specificity of the prepared monoclonal antibody with direct ELISA (A) and competitive ELISA (B). In (A), black circles (•) indicate reaction by mAb 92–99 against synthesized β2m 92–99 peptide, triangle mark (▵) indicates reaction by polyclonal anti-β2m serum against synthesized β2m 92–99 peptide, (×) mark indicates a reaction by β2m-monoclonal antibody 4559 against synthesized β2m 92–99 peptide. In (B), black circles (•) indicate competition with the synthetic peptide, triangle mark (▵) indicates competition with the acidified-β2m, and square mark (▪) indicates competition with the re-β2m.
Kidney International  , DOI: ( /j x)
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4. Figure 3
Immunoblotting for various specimens. Serum (A), acidified β2m of ultrafiltrate from HD patient's plasma (B), β2m of the ultrafiltrate from HD patient's plasma (C), homogenate from carpal tissue (D and E).
Kidney International  , DOI: ( /j x)
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5. Figure 4
Circular dichroism pattern of β2m isolated from plasma ultrafiltrate. Dotted line indicates pH 7.0 (a loss of the peak at 205nm), and solid line indicates pH 3.0 (a shift of the minimum at 218nm).
Kidney International  , DOI: ( /j x)
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6. Figure 5
Immunostaining and histologic study for amyloid deposist in carpal tissue from HD patient (65 years old, female, 14 years of HD duration). Immunostaining polyclonal anti-β2m serum (A). Both the area indicated by the square frame and by the arrowhead show an accumulation of β2m. Congo red staining (B). An area indicated by the square frame shows a distinct red color. Polarizing microscopy of Congo red staining (C). An area indicated by the square frame shows a distinct yellow-green birefringence, but an area indicated by the arrowhead in (B) shows no polarized light at all. Immunostaining with mAb 92–99 (D). An area indicated by the square frame shows a presence of β2m with unfolded C-terminus. By contrast, an area indicated by the arrowhead shows a lacking of β2m with unfolded C-terminus.
Kidney International  , DOI: ( /j x)
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7. Figure 6
Immunostaining and histologic study for amyloid deposist in corocoacrominal ligament from an HD patient 38 years old, male, 19 years of HD duration (A), and an HD patient 55 years old, female, 12 years of HD duration (B). Congo red stain: negative, mAb92-99 stain: positive (A); Congo red stain: negative, mAb 92–99 stain: negative (B).
Kidney International  , DOI: ( /j x)
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