1. Lipid presentation by CD11c+ cells controls iNKT cell subsets
Lipid presentation by CD11c+ cells controls iNKT cell subsets A, BAnalysis of iNKT cells populations in the SI‐LP (A) and mLN (B) from CD1dfl/flCD11cCre mice, showing flow cytometry plots and frequency of total iNKT cells (grey) and NKT1 (RORγt−PLZFloT‐bet+; green), NKT2 (RORγt−PLZFhiT‐bet−; red), NKT17 (PLZFintRORγt+; blue) and PLZFloT‐bet− NKT cells (orange; mean ± SEM). Data are from 4–6 experiments.C–EC57BL/6 WT mice were orally gavaged with αGalCer, and iNKT cell populations were analysed 3 days later. Flow cytometry plots (C), frequency (D) and total number (E) of iNKT cells and NKT1, NKT2 and NKT17 cells in the mLN are shown. Data are from three experiments.FHistograms showing Ki‐67 expression (left) and frequency of Ki‐67+ (right) NKT1, NKT2 and NKT17 cells in the mLN from C57BL/6 WT mice 2 days after oral αGalCer administration.G, HFrequency (G) and total number (H) of iNKT cells and NKT1, NKT2 and NKT17 cells in the mLN from Cre+ and Cre− CD1dfl/flCD11cCre mice 3 days after oral αGalCer administration. Data are from 2–3 experiments.Data information: Numbers indicate percentage of cells in the indicated gates. Each dot in the bar plots is an individual mouse and bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, two‐tailed unpaired t‐test.
Julia Sáez de Guinoa et al. EMBO J. 2018;37:e97537
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