1. Figure 1. Outer membrane protein profiling of parental strain KJΔDEFΔ5 and its derivative fluoroquinolone-resistant ...
Figure 1. Outer membrane protein profiling of parental strain KJΔDEFΔ5 and its derivative fluoroquinolone-resistant mutants C2204, C2205, C2206, L1209, L1301 and L1305. Outer membrane proteins were prepared as described in the Materials and methods section. Equal protein concentrations were separated by SDS-PAGE with a 5% stacking gel and a 15% separating gel, and stained with Coomassie brilliant blue. Lane M, molecular weight standards. The black arrow indicates the protein excised for LC-MS/MS analysis.
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2. Figure 2. Expression of efflux pump genes of KJΔDEFΔ5 and its derivative MDR mutants. Bacterial cells that had been ...
Figure 2. Expression of efflux pump genes of KJΔDEFΔ5 and its derivative MDR mutants. Bacterial cells that had been cultured overnight were inoculated into fresh LB broth with an initial OD₄₅₀ nm of After 5 h incubation, the transcripts were quantified by RT-qPCR. All values were normalized to the transcript level obtained from KJΔDEFΔ5. Bars represent the average values from three independent experiments. Error bars represent the SEM. *P <0.001, calculated by Student’s t test. (a) tolCsm transcript in KJΔDEFΔ5 and its derivative MDR mutants C2204, C2205, C2206, L1209, L1301 and L1305. (b) smeH, smeK, smeN, smeP and smeZ transcripts in KJΔDEFΔ5 and its derivative MDR mutants C2206, L1301 and L1305.
Unless provided in the caption above, the following copyright applies to the content of this slide: © The Author(s) Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (
J Antimicrob Chemother, dkz200,
The content of this slide may be subject to copyright: please see the slide notes for details.