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Modification of the human allergic immune response by allergen-DNA–transfected dendritic cells in vitro  Bettina Klostermann, MSc, Iris Bellinghausen,

Published byLiana Sumadi Modified over 5 years ago

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Presentation on theme: "Modification of the human allergic immune response by allergen-DNA–transfected dendritic cells in vitro  Bettina Klostermann, MSc, Iris Bellinghausen,"— Presentation transcript:

1 Modification of the human allergic immune response by allergen-DNA–transfected dendritic cells in vitro  Bettina Klostermann, MSc, Iris Bellinghausen, PhD, Ingo Böttcher, MSc, Arnd Petersen, PhD, Wolf-Meinhard Becker, PhD, Jürgen Knop, MD, Joachim Saloga, MD  Journal of Allergy and Clinical Immunology  Volume 113, Issue 2, Pages (February 2004) DOI: /j.jaci

2 FIG 1 Efficiency of the adenoviral transfection of human mature DCs. DCs were generated as described and transfected at day 6 with adenoviral vectors encoding green fluorescent protein at different MOIs. One representative example of 8 independent experiments is shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

3 FIG 2 Expression of the protein Phl p 1 by transfected DCs. The cells were lysed 48 hours after transfection. Phl p 1 protein (0.5 ng) was used as a positive control, and as a negative control, we used untransfected mature DCs. A representative example of 3 separate experiments is shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

4 FIG 3 Surface marker expression of DCs after adenoviral transfection or exposure to allergen protein. DCs were generated as described and transfected with Ad-Phl p 1 at day 6. After 48 hours, cells were stained with the markers indicated. A representative example of 3 separate experiments is shown. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

5 FIG 4 Proliferation of autologous human CD4+ (A) and CD8+ (B) T cells activated by transfected DCs. T cells were stimulated with allergen protein–pulsed, transfected, or control DCs. After 5 days, cells were pulsed with 1 μCi of tritiated thymidine. Results are expressed as the means ± SD from 8 atopic donors. ∗P ≤ .05 compared with medium or Psi 5. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

6 FIG 5 Cytokine production by CD4+ (A) and CD8+ (B) T cells after stimulation with autologous DCs. T cells were stimulated twice with allergen protein–pulsed, transfected, or control DCs, and cytokines were measured by means of ELISA. Results are expressed as means ± SD from 6 donors. ∗P ≤ .05 compared with medium or Psi 5. †P ≤ .03 comparing allergen-DNA conjugate– transfected and allergen protein–pulsed DCs. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

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