1. Consistent responses of the human vascular smooth muscle cell in culture: Implications for restenosis 
Euan Munro, FRCSEd, Philip Chan, FRCS, Mahendra Patel, PhD, Laura Betteridge, BSc(Hons), Karen Gallagher, BSc(Hons), Michael Schachter, MRCP, Peter Sever, FRCP, John Wolfe, FRCS 
Journal of Vascular Surgery 
Volume 20, Issue 3, Pages (September 1994)
DOI: / (94)90149-X
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2. Fig. 1
A, Mean (±range) percentage of 14-day proliferation of 15% FCS stimulated VSMC at passage 3 derived from proximal (diagonal bars), middle (unfilled bars), distal (checkered bars), and pooled (filled bars) cultures of saphenous vein from seven patients. Fourteen-day percentage proliferation was calculated by ([cell count at day 14/cell count at day 0] × 100). Variations between cultures were not significantly different from interreplicate variance (experimental error) (ANOVA). VSMC proliferation rates did not differ significantly between individual patients (ANOVA). B, Mean (± range) percentage of 14-day heparin inhibition of proliferation of 15% FCS stimulated VSMC at passage 3 derived from proximal (diagonal bars), middle (unfilled bars), distal (checkered bars), and pooled (filled bars) cultures of saphenous vein from seven patients. Fourteen-day heparin inhibition of proliferation was calculated by ([1 - (cell count of heparin-treated cells at day 14/cell count of control cells at day 14]) × 100. Differences between cultures were not significantly different from interreplicate variance (experimental error) (ANOVA), but see results regarding patient 3. Heparin inhibition was significantly different between individual patients (p = 0.02, ANOVA).
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3. Fig. 1
A, Mean (±range) percentage of 14-day proliferation of 15% FCS stimulated VSMC at passage 3 derived from proximal (diagonal bars), middle (unfilled bars), distal (checkered bars), and pooled (filled bars) cultures of saphenous vein from seven patients. Fourteen-day percentage proliferation was calculated by ([cell count at day 14/cell count at day 0] × 100). Variations between cultures were not significantly different from interreplicate variance (experimental error) (ANOVA). VSMC proliferation rates did not differ significantly between individual patients (ANOVA). B, Mean (± range) percentage of 14-day heparin inhibition of proliferation of 15% FCS stimulated VSMC at passage 3 derived from proximal (diagonal bars), middle (unfilled bars), distal (checkered bars), and pooled (filled bars) cultures of saphenous vein from seven patients. Fourteen-day heparin inhibition of proliferation was calculated by ([1 - (cell count of heparin-treated cells at day 14/cell count of control cells at day 14]) × 100. Differences between cultures were not significantly different from interreplicate variance (experimental error) (ANOVA), but see results regarding patient 3. Heparin inhibition was significantly different between individual patients (p = 0.02, ANOVA).
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4. Fig. 2
Cell number (mean ± range) of 15% FCS stimulated VSMC at passage 3 derived from proximal, middle, distal, and pooled cultures of saphenous vein from single patient over 14 days.
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5. Fig. 3
A, Mean (± range) 14-day percentage proliferation of 15% FCS stimulated VSMC derived from pooled cultures of saphenous vein from six patients at passages 2 or 3 to 6. Fourteen-day percentage proliferation was calculated by ([cell count at day 14/cell count at day 0] × 100). Variations in VSMC proliferation rates at different passages for individual patients were not significantly different from interreplicate variance (experimental error) (ANOVA). B, Mean (± range) 14-day heparin inhibition of proliferation of 15% FCS stimulated VSMC derived from pooled cultures of saphenous vein from six patients at passages 2 or 3 to 6. Fourteen-day heparin inhibition of proliferation was calculated by ([1 - [cell count of heparin treated cells at day 14/cell count of control cells at day 14]) × 100. Variations in heparin inhibition at different passages within each patient were not significantly different from interreplicate variance (experimental error) (ANOVA).
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

6. Fig. 3
A, Mean (± range) 14-day percentage proliferation of 15% FCS stimulated VSMC derived from pooled cultures of saphenous vein from six patients at passages 2 or 3 to 6. Fourteen-day percentage proliferation was calculated by ([cell count at day 14/cell count at day 0] × 100). Variations in VSMC proliferation rates at different passages for individual patients were not significantly different from interreplicate variance (experimental error) (ANOVA). B, Mean (± range) 14-day heparin inhibition of proliferation of 15% FCS stimulated VSMC derived from pooled cultures of saphenous vein from six patients at passages 2 or 3 to 6. Fourteen-day heparin inhibition of proliferation was calculated by ([1 - [cell count of heparin treated cells at day 14/cell count of control cells at day 14]) × 100. Variations in heparin inhibition at different passages within each patient were not significantly different from interreplicate variance (experimental error) (ANOVA).
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

7. Fig. 4
A, Mean (± range) 14-day percentage proliferation of 15% FCS stimulated VSMC derived from paired artery (filled bars) and saphenous vein (unfilled bars) samples from four patients. Fourteen-day percentage proliferation was calculated by ([cell count at day 14/cell count at day 0] × 100). Variations in proliferation rates between artery and vein samples within each pair were not significantly different from interreplicate variance (experimental error) (ANOVA). B, Mean (± range) 14-day heparin inhibition of proliferation of 15% FCS stimulated VSMC derived from paired artery (filled bars) and saphenous vein (unfilled bars) samples from four patients. Fourteen-day heparin inhibition of proliferation was calculated by (1 - [cell count of heparin treated cells at day 14/cell count of control cells at day 14]) × 100. Variations between heparin inhibitions of artery and vein samples within each pair were not significantly different from interreplicate variance (experimental error) (ANOVA).
Journal of Vascular Surgery  , DOI: ( / (94)90149-X)
Copyright © 1994 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions