1. GARLIC & HEALTH WP2: breeding Embryogenesis Half year meeting Munich
30 sept.-2 oct. 2003
WP2: breeding
Embryogenesis

2. Objectives Embryogenesis WP2: breeding
Setting a somatic embryogenesis procedure
Comparative characterisation of plants from somatic embryo-genesis and from classic micro-propagation after meristem culture (as control)

3. Work plan
Establishment of somatic embryogenesis process for : Rouge de La Réunion, Messidrôme, Morasol & Printanor
Production and provision of embryogenic cell suspension cultures of these varieties
Genetic evaluation of somatic embryo derived plants through
flow cytometry (P8),
biochemistry (P5),
molecular markers (P1),
morphological and physiological analyses (P8, P9)

4. Varietal effect on somatic embryogenesis from cell suspension culture

5. Deliverables
DP 5: Evidence of a somatic embryogenesis process and plant regeneration and acclimatisation in garlic (Allium sativum L.)
L. FEREOL, V. CHOVELON, S. CAUSSE, N. MICHAUX FERRIERE and R. KAHANE, 2002.
Plant Cell Reports 21:
DP13: Establishment of garlic (Allium sativum L.) cell suspensions and plant regeneration
L. FEREOL, V. CHOVELON, S. CAUSSE, D. TRIAIRE, N. MICHAUX FERRIERE and R. KAHANE
Submitted to Plant Science in June 2003
DP21: paper on the use of somatic embryogenesis for rapid multiplication of garlic in vitro.
In progress (to submit to In Vitro Cell. Dev. Biol.)

6. What will be done till 2004 Paper DP 21
Embryogenic cell suspension cultures as A method for garlic (Allium sativum L.) mass propagation
Characterising the embryogenic process :
Histological studies: achieved
Cytometric studies: running
No maintenance of the material
Field evaluation : running

7. CIRAD Embryogenesis WP2: breeding L. Féréol S. Causse M. Roux-Cuvelier
somatic embryogenesis, histology and flow cytometry,
paper writing
S. Causse
technical assistant
somatic embryogenesis, histology and flow cytometry
M. Roux-Cuvelier
field trials in La Réunion
R. Kahane
paper revision, management