1. Absence of a retinal phenotype in CIB2−/− mice
Absence of a retinal phenotype in CIB2−/− mice AElectroretinogram (ERG) recordings in 3‐month‐old CIB2+/− (black) and CIB2−/− (red) mice.BQuantification of scotopic a‐wave (rod hyperpolarization) and b‐wave (bipolar neuron depolarization) ERG amplitudes, and photopic ERG amplitudes (cone pathway), showing no significant difference between the two genotypes. The data shown are means ± SEM, n = 16 for CIB2+/− mice and n = 20 for CIB2−/− mice; Student's t‐test; “ns” indicates a statistically non‐significant difference, P > 0.1).C, DNo difference in retinal layer organization was observed between CIB2+/− and CIB2−/− mice at 3 months as illustrated by DAPI and F‐actin staining (C), and no TUNEL‐positive cell was observed in CIB2−/− (red) mice at 9 months (D).E, FThe Iba1‐immunoreactive microglial cells are evenly distributed in the inner (IPL) and outer (OPL) plexiform layers in both CIB2+/− (E) and CIB2−/− (F) mice.G, HThe scanning electron microscopy micrographs of the inner (IS)‐outer (OS) segment interface show normal architecture in CIB2+/− (G) and CIB2−/− (H) mice at 9 months. The immunostaining for rhodopsin and blue opsin reveals no difference between the two genotypes, with the opsin staining normally restricted to unaffected outer segments.Data information: Scale bars: 20 μm (C–F), 2 μm (G, H). ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer.
Vincent Michel et al. EMBO Mol Med. 2017;9:
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