1. Impact of phosphatase deletion on archaella expression and motility.
Impact of phosphatase deletion on archaella expression and motility.A, For the nutrient limitation assay MW001, Δsaci_ptp and Δsaci_pp2a were grown to an OD600 of about 0.4 in Brock medium supplemented with 0.1% NZ-amine, 0.2% sucrose and 10 μg/ml uracil and then transferred to medium lacking the nutrient source. After 6 h samples were collected and immunoblotting was performed with specific antibodies against the archaellum proteins FlaB and FlaX. The amount of protein in Δsaci_ptp is comparable to the strain MW001, whereas for Δsaci_pp2a highly elevated expression of FlaB and FlaX was observed after the starvation stress. Interestingly, already before stress induction the FlaB levels were increased in the Saci-PP2A deletion mutant. B, The wild type strain MW001, the two phosphatase deletion strains Δsaci_ptp and Δsaci_pp2a, the hypermotile positive control strain ΔaapF and the negative nonmotile strain ΔaapFΔflaH were spotted on semi-solid Gelrite dishes and incubated for 5 days at 76 °C. Only a small halo is visible for MW001 and Δsaci_ptp, whereas Δsaci_pp2a was as hypermotile as the positive control strain.
Julia Reimann et al. Mol Cell Proteomics 2013;12:
© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.