1. Getting the YSI Sonde Ready For Use

2. Quality Data Proper Maintenance & Calibration
Keep all electrical contacts dry
Proper removal / installation of probes
Good DO membrane installation
Use Good Laboratory Practice
Proper Rinse method between buffers/standards
Pre-rinse…using small amount of calibration solution
Allowing time for Equilibration
Quality Calibration
Understanding Sensor Diagnostics

3. Probe Installation/Removal Review Handout
DISSOLVED OXYGEN
ISE 4
ISE 3 4 3 5
CHLOROPHYLL
TURBIDITY C T
ISE1 / ISE2
(pH / ORP)
COND/TEMP
ISE 5

4. Reconditioning DO Probe for Maintenance and/or High DO Charge
Remove membrane, rinse probe in clean water, and dry off probe surface
Use the YSI 6035 kit to service electrodes (note that the sanding disc must be stroke parallel with the electrodes) (Care & Maintenance Section)
Rinse probe surface area with clean water and wipe away residue
Re-membrane probe and check DO Charge (after 5 minutes of probe burn-in). NOTE: Install new O-RING
If reconditioning DO Probe does not reduce High Charge, repeat conditioning. If it does not help, then replace probe or contact Technical Support at YSI for further assistance.

5. DO Probe Service
2 Dirty Anodes
1 Dirty Anode
Serviced Probe

6. Membrane Installation / Quality Data Prepare the Probe with KCl

7. Prepare to Install Membrane

8. Finish Installation of Membrane

9. Keep Membrane Tight to Install O-Ring

10. Cut Excess Material Away

11. DO Probe Ready to Proceed

12. Precision Certified Thermometer
Temperature
No calibration or service available
But check accuracy with a
Precision Certified Thermometer

13. Conductivity Service and Calibration
Always service conductivity cell before calibration (Care and Maintenance Section)
Always use a standard that is a 1000 uS/cm or greater
Pre-rinse before calibration
Always use fresh standards, and allow time to equilibrate
Ensure that conductivity cell is totally submerged in solution and lightly shake sonde to remove any air bubbles
Never accept an “Out of Range”

14. Conductivity Probe Service

15. Conductivity Calibration Precautions & Diagnostics
Calibrate in SpCond (Specific Conductance)
Enter correct value e.g.: 1000 uS/cm Calibration value has to be entered as a mS/cm) uS/cm = 1 mS/cm
After calibration “ALWAYS” check “AND” record Cell Constant in “Advanced / Cal Constants Menu” Range 5.0 ± 0.5 (should be very close with each cal.)
If Cell Constant is out of range clean and recalibrate
SEE CALIBRATION WORK SHEET

16. pH/ORP Maintenance Tips
During calibration; if mV readings are out of specs, or if there is slow response, do cleaning procedure to probe (Care and Maintenance Section)
Soak probe for hrs. in 2M KCl note: Proper Storage will extend the life of the probe
If all this fails, replace electrode (Reference first)

17. pH Probe Service
Clean pH Probe
Dirty pH/ORP Probe

18. pH / ORP Diagnostics and Calibration
In “Report Menu” enable pH mV
Place into a pH 7.0 buffer, let Equilibrate and record mV value. Considered acceptable range: 0 +/- 50 ??
4.0 buffer should be +177 (from 7 buffer mV value) range  165 to 180 mV
10.0 buffer should be -177 (from 7 buffer mV value) range  -165 to -180 mV

19. pH The potential is related to the pH by a form of the Nernst Equation
pH mV
pH mV
pH mV

20. pH Calibration Tips
Calibrate using at least a 2 Point Cal (7 buffer always being the first point)
Make sure Temperature Probe is in the pH Buffer and allowing time for Equilibration
Pre-rinse between buffers
Record mV values in all buffers and check probe response between buffers
Never accept an “Out of Range”
SEE CALIBRATION WORK SHEET

21. ORP / REDOX Calibration
CALIBRATION CHART
Temperature Celsius Zobell Solution Value, MV
NOTE: All ISE sensors, requires the pH and temperature
probe to be in solution during calibration.
Never accept an “Out of Range”
SEE CALIBRATION WORK SHEET

22. Depth Sensor Maintenance
Use syringe from Maintenance Kit; this is the only tool to be used on the depth sensor
If further cleaning is required, soak in warm soapy tap water, then try syringe again

23. Depth Sensor Calibration
Calibrate in air to zero
For more accurate reading in reference to WQ sensors; apply offset to calibration
Never accept an “Out of Range”
SEE CALIBRATION WORK SHEET

24. DO Sensor Diagnostics
In Report menu; Enable DO Charge confirm that DO charge is 50 +/- 25, if out of specs recondition probe
Put into RUN MODE for minutes before calibration (Probe Burn In)
Confirm DO Warm-up Pattern (PROBE HAS TO BE COLD BEFORE STARTING THIS TEST)
Check DO Gain “Advance/Cal Const. Menu” (After Calibration) should be 1 within a range of .7 to 1.5

25. Dissolved Oxygen Calibration
Calibrate with probe cold OR fully warmed up ?? (RS232 auto sleep enabled) ; UNATTENDED (RS232 auto sleep disabled) ; SPOT SAMPLING
Membrane and temperature sensor must be dry
Probe must be in a water saturated air, or air saturated water
Enter current barometer reading (mm/Hg)
Never accept an “Out of Range”
SEE CALIBRATION WORK SHEET

26. Optic Probes Service and Calibration
Turbidity
Chlorophyll
Rhodamine
BGA-PC…Fresh to Brackish Water
BGA-PE…Brackish to Marine Water DO

27. Dirty Optic Wiper Pads

28. Clean Optic Wiper Pads

29. Optic Probe Wiper
Wiper Motor
Wiper Sponge
Wiper

30. Proper Spacing for Wiper Block

31. Turbidity Probe test and Calibration
Finger Test to confirm probe response and wiper parking
Calibration process more critical in certain applications: (review handout)
Standards to use / value to enter (review handout)
For 6600EDS Sonde; remove brush and install clean wiper block with clean pad

32. Chlorophyll, Rhodamine and BGA Calibration
Accept default calibration and use for screening and/or monitoring trends
Check for drift and/or slightly enhance accuracy using dye solution to simulate field data
Use extractive analysis results from field samples to post calibrate all readings (Chlorophyll, BGA)

33. Post-calibration of In Vivo Chl Readings

34. Calibration of Optical DO
Calibration method the same as for Rapid Pulse, e.g. air saturated water / water saturated air, entering correct barometric pressure value, etc (RS232 can be enabled for either application)
Can do a 2 point calibration; 1st. Point being in zero oxygen environment; 2nd. Point in saturated environment
Diagnostics is DO gain; acceptable range is .75 to 1.25
MIKE
Testing has shown the sensor drift is very minimal with the ROX system.
Drift has been so minimal that when a drift is observed the user is strongly advised to double check their setup.

35. Membrane Removal
The wiper set screw is loosened with the Allen tool provided, then the wiper is removed.

36. Membrane Removal The membrane can now be removed.
The three membrane screws are removed using the Allen tool provided.
MIKE

37. Membrane Replacement
Remove the new sensor membrane assembly from its hydrated container and dry the underside completely. Ensure both sides of the membrane cavity are dry.
Be sure to enter the new calibration code values into sonde menu.
MIKE
Topside
Underside

38. Final test of all Sensors after Calibration
Place sensors in tap water (aerated for 20 minutes or more)
Check for response time to stabilize on all sensors (this will vary from sensor to sensor), and look for realistic values
Review handout on pH response test

39. REVIEW
CALIBRATION WORK SHEET
“GLP” FILE
TRANSPORT METHODS OF SONDE

40. Sensor Storage Recommendations
“General Rule”
Short term (4 weeks or less): Saturated Air
Long term (Seasonal): Varies with Sonde Model
2. Sonde Care and Maintenance Review Handout

41. Questions….