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Kaihui Liang, Nosratola D. Vaziri  Kidney International 

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Presentation on theme: "Kaihui Liang, Nosratola D. Vaziri  Kidney International "— Presentation transcript:

1 Down-regulation of hepatic high-density lipoprotein receptor, SR-B1, in nephrotic syndrome 
Kaihui Liang, Nosratola D. Vaziri  Kidney International  Volume 56, Issue 2, Pages (August 1999) DOI: /j x Copyright © 1999 International Society of Nephrology Terms and Conditions

2 Figure 1 (Upper panel) Representative Western blot of HDL receptor (HDLR) protein obtained in a placebo-treated rat (NL), a pre-nephrotic rat studied 24 hours after puromycin aminonucleoside (PAN) injection (D1), and the nephrotic animals studied on days 14 (D14) and 30 (D30) after initial PAN injection. (Lower panel) Group data reflecting relative optical densities of HDLR protein bands in the corresponding subgroups. N = 6 in each subgroup. *P < 0.01 vs. vales found in NL and D1 subgroups. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

3 Figure 2 (Upper panel) Representative Northern blot of hepatic HDL receptor (HDL-R) mRNA and the corresponding actin mRNA in two nephrotic rats (NS) and two normal control rats (NL) studied 30 days after initial injections of puromycin aminonucleoside (PAN) or placebo, respectively. (Lower panel) Group data illustrating the hepatic HDL-receptor/actin mRNA in six nephrotic (NS) and six normal control (NL) rats obtained 30 days after initial injections of PAN and placebo, respectively. No significant difference was observed between the two groups. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

4 Figure 3 (Upper panel) Representative Northern blots of hepatic apo A-I mRNA and actin mRNA in two nephrotic (NS) and two normal control (NL) animals studied 30 days after the initial injections of puromycin and placebo, respectively. (Lower panel) Group data depicting hepatic apo A-I/actin mRNA in nephrotic rats (NS) studied 30 days after initial injections of puromycin (N = 6) and normal controls (NL) studied 30 days after placebo injections (N = 6). *P < 0.01. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions

5 Figure 4 (A) Hepatic apo A-I/actin mRNA (ratio). (B) Hepatic high-density lipoprotein (HDL) receptor/actin mRNA (ratio) in rats studied 24 hours after intraperitoneal injections of puromycin aminonucleoside, 130 mg/kg (PAN), or vehicle (NL) alone. No significant difference was observed between the two subgroups. N = 6 in each subgroup. Kidney International  , DOI: ( /j x) Copyright © 1999 International Society of Nephrology Terms and Conditions


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