1. Volume 26, Issue 9, Pages 1221-1228 (May 2016)
Organelle Size Scaling of the Budding Yeast Vacuole by Relative Growth and Inheritance 
Yee-Hung M. Chan, Lorena Reyes, Saba M. Sohail, Nancy K. Tran, Wallace F. Marshall 
Current Biology 
Volume 26, Issue 9, Pages (May 2016)
DOI: /j.cub
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2. Figure 1
Vacuole Inheritance Defect Causes Divergence in Vacuole-to-Cell Size Scaling
(A) Representative images of wild-type and vac8Δ cells (bright-field) and vacuoles (Vph1p-GFP). In each image, the cells are arranged in a frequently observed pattern where a second-generation and later mother, M, has produced a first-generation daughter, D, in the previous cell cycle, and is currently producing a new bud, B.
(B and C) Vacuole volume (B) and vacuole surface area (C) versus cell volume scaling plots for wild-type (blue) and vac8Δ (red) cells. Wild-type and vac8Δ scaling plots are significantly different from one another for both volume and surface area (p < 0.001, comparison of regression lines).
See also Figure S1.
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3. Figure 2
Vacuole Size Scaling Defects in Inheritance Mutants Increase with Replicative Age
(A–D) Wild-type vacuole surface area (A), wild-type vacuole volume (B), vac8Δ vacuole surface area (C), and vac8Δ vacuole volume (D) versus cell volume scaling plots were separated according to generational age. Lines represent best linear fits (solid, p < 0.05; dashed, p > 0.05).
(E and F) Vacuole surface area-to-cell volume (E) and vacuole volume-to-cell volume (F) ratios were averaged for cells binned by replicative age. Lines represent best linear fits, and error bars represent SDs. vac8Δ cells (red) show a much steeper increase in scaling ratio than wild-type (blue).
See also Figure S2.
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4. Figure 3
Wild-Type and vac8Δ Vacuoles Grow at Similar Rates Regardless of the Vacuole-to-Cell Size Scaling Ratio
(A) Total cell (mother cell and its bud) and total vacuole sizes were measured at 10-min intervals for first-generation mothers undergoing their first G1 (blue shading) and budding (red shading) followed by their second cell cycle (no shading).
(B–K) Time course plots show average values at each time point with error bars depicting SDs.
(B and C) Wild-type (B; n = 10) and vac8Δ (C; n = 10) average cell volume plotted against time.
(D and E) Wild-type (D) and vac8Δ (E) average vacuole surface area plots.
(F and G) Wild-type (F) and vac8Δ (G) average vacuole volume plots.
In (D) and (F), the cyan line corresponds to the sum of vacuole sizes in the now second-generation mother and her newborn first-generation daughter. This represents the cumulative amount of vacuole growth.
(H and I) Vacuole surface area-to-cell volume ratios were calculated and averaged for each time point for wild-type (H) and vac8Δ (I) cells.
(J and K) Vacuole volume-to-cell volume ratios were calculated and averaged for each time point for wild-type (J) and vac8Δ (K) cells.
See also Figures S3 and S4.
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5. Figure 4 Model of Cell Growth, Vacuole Growth, and Vacuole Inheritance
(A) Illustration of the simulation of cell and vacuole growth. In G1, a mother cell and its vacuole grow. The mother enters the cell cycle in which growth is restricted to the bud. When the bud reaches a certain size, division can occur, producing two mothers that enter another iteration of the cell cycle.
(B) Using constant cell and vacuole growth rates produces populations that are similar to wild-type, and increasing variance in these rates leads to decreasing R2 coefficients in the population scaling plots.
(C) Vacuole growth can be localized to the bud (left; wild-type), mother (vac8Δ; right), or randomly distributed between both cells (middle). Changes to inheritance lead to divergence in the scaling plots with respect to replicative age.
See also Table S1.
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