1. Application of complementary DNA microarray (DNA chip) technology in the study of gene expression profiles during folliculogenesis 
Hung-Ching Liu, Ph.D., Zhiming He, M.S., Zev Rosenwaks, M.D. 
Fertility and Sterility 
Volume 75, Issue 5, Pages (May 2001)
DOI: /S (01)01706-X

2. Figure 1
In vitro maturation of preantral follicles: mechanically isolated preantral follicles were matured in vitro as described in text. This figure depicts various stages during maturation and early embryo development. (A) Nonstimulated preantral follicle; (B) granulosa cell proliferation; (C) antral formation; (D) ovulation; (E) MII oocytes; and (F) blastocyst-stage embryo. Nonstimulated preantral follicles (in A) collected on day 0 and stimulated Graafian follicles (in C) collected on day 10 were subjected for RNA extraction for gene expression analysis by DNA chip technology.
Liu. Gene expression during folliculogenesis. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)01706-X)

3. Figure 2
Gene expression profiles on DNA expression chips. The digital images of prestimulated preantral follicles were illustrated. (A) Prestimulated Graafian follicles; (B) stimulated Graafian follicles; and (C) superimposed image of both profiles, evaluated by Borland Delphi 3.0 software.
Liu. Gene expression during folliculogenesis. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)01706-X)

4. Figure 3
Distribution and comparison of gene expression levels. Thirty-nine and 61 genes were detected in preantral and Graafian follicles, respectively. Distribution of these identified genes according to their expression levels was analyzed.
Liu. Gene expression during folliculogenesis. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)01706-X)

5. Figure 4
Clustering analysis of identified genes. Identified genes were clustered according to the log values of Graafian (G)/preantral (P) gene expression. Fifteen genes were found to be down-regulated (log G/P <0), and 46 were significantly up-regulated (log G/P >0).
Liu. Gene expression during folliculogenesis. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)01706-X)

6. Figure 5
Number of up-regulated and down-regulated genes. Of 15 down-regulated genes, 2 decreased more than 3-fold, and 13 decreased 1- to 3-fold; and of 46 up-regulated genes, 22 increased 1- to 3-fold, 5 increased 3- to 10-fold, 14 increased 10- to 100-fold, and 5 increased >100-fold.
Liu. Gene expression during folliculogenesis. Fertil Steril 2001.
Fertility and Sterility  , DOI: ( /S (01)01706-X)