1. Using divisional history to measure hematopoietic stem cell self-renewal and differentiation 
Feng Yan, Michael I Collector, Sara Tyszko, Saul J Sharkis 
Experimental Hematology 
Volume 31, Issue 1, Pages (January 2003)
DOI: /S X(02)

2. Figure 1
Sorting of FR25Lin−PKH+ bone marrow cells. FR25 cells were labeled with lineage antibodies and PKH-26. The gate of lineage-negative and PKH-positive cells was defined for sorting FR25Lin−PKH+ cells.
Experimental Hematology  , 56-64DOI: ( /S X(02) )

3. Figure 2
Profiles of PKHbright and PKHdull cells on day 5 and day 7 in culture. The PKHbright cells were defined as the cells that had the same PKH intensity as day-0 PKH+ cells. The PKHbright cells and PKHdull cells were sorted as gated in the above panels. PKHdull cells were defined as the cells that have less intensity of PKH as day-0 PKH+ cells.
Experimental Hematology  , 56-64DOI: ( /S X(02) )

4. Figure 3
Long-term engraftment of various doses of fresh FR25Lin− cells (A), day-5 PKHbright and PKHdull cells (B), and day-7 PKHbright and PKHdull cells (C) as detected in the blood of recipients by FISH for the Y-chromosome at 3 and 6 months posttransplantation. Data were from 2 (fresh FR25Lin− cells), 3 (day-5 cells) and 4 (day-7 cells) experiments. *: p < 0.05 between 3 and 6 months. **: p < 0.05 between day 5 and day 7 at both 3 and 6 months.
Experimental Hematology  , 56-64DOI: ( /S X(02) )

5. Figure 4
HSC fates after divisions. (A,B) Possible patterns of HSC division. (A) Asymmetric divisions result in one stem cell and one differentiated cell after each division. After three divisions, only one stem cell remained and more progenitors were produced. This type of division leads to HSC maintenance only. S indicates stem cell; P, progenitor cell. (B) Symmetric divisions result in either two stem cells or two differentiated cells after each division. If the possibility of these two types of divisions was equal in a population, after three divisions, two starting stem cells remained and the outcome of an HSC division would be asymmetric. HSC expansion only occurs when symmetric stem cell divisions predominate (greater than 50% of the divisions) in a population. (C) Cell fates of FR25Lin−PKH+ cells during in vitro culture.
Experimental Hematology  , 56-64DOI: ( /S X(02) )