Presentation is loading. Please wait.

Presentation is loading. Please wait.

Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA  Hidekazu Horiuchi, Ayako Sasaki, Motoki.

Published byGunn Austad Modified over 5 years ago

Similar presentations

Presentation on theme: "Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA  Hidekazu Horiuchi, Ayako Sasaki, Motoki."— Presentation transcript:

1 Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA  Hidekazu Horiuchi, Ayako Sasaki, Motoki Osawa, Kazuki Kijima, Yukiko Ino, Ryoji Matoba, Kiyoshi Hayasaka  The Journal of Molecular Diagnostics  Volume 7, Issue 5, Pages (November 2005) DOI: /S (10) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Polyacrylamide gel electrophoresis of typical PCR products obtained for the polyalanine tract of PHOX2B using the deaminated DNA as a template. The gel is stained with ethidium bromide. Lane L, 10-bp ladder; lane 1, homozygous 20-residue repeat; lane 2, heterozygous 13-residue repeat; lane 3, heterozygous 15-residue repeat; lane 4, homozygous 15-resiude repeat; and lanes 5 to 14, the 10 CCHS cases corresponding to those in the previous analysis.5 The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

3 Figure 2 Nucleotide sequence of the converted DNA at the common 20-residue polyalanine tract. The sequence of a subcloned fragment is shown to demonstrate the replacement of C to T more clearly. Arrows indicate the converted T after bisulfite treatment. The Journal of Molecular Diagnostics 2005 7, DOI: ( /S (10) ) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Download ppt "Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA  Hidekazu Horiuchi, Ayako Sasaki, Motoki."

Similar presentations

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.

Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.

Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.

Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.
An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.

An Enhanced Polymerase Chain Reaction Assay to Detect Pre- and Full Mutation Alleles of the Fragile X Mental Retardation 1 Gene  Alessandro Saluto, Alessandro.
Translational Genomics to Develop a Salmonella enterica Serovar Paratyphi A Multiplex Polymerase Chain Reaction Assay  Hong-Yu Ou, Cindy Teh Shuan Ju,

Translational Genomics to Develop a Salmonella enterica Serovar Paratyphi A Multiplex Polymerase Chain Reaction Assay  Hong-Yu Ou, Cindy Teh Shuan Ju,
A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.

A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.
Large Pathogenic Expansions in the SCA2 and SCA7 Genes Can Be Detected by Fluorescent Repeat-Primed Polymerase Chain Reaction Assay  Claudia Cagnoli,

Large Pathogenic Expansions in the SCA2 and SCA7 Genes Can Be Detected by Fluorescent Repeat-Primed Polymerase Chain Reaction Assay  Claudia Cagnoli,
Detection of Exon 12 Mutations in the JAK2 Gene

Detection of Exon 12 Mutations in the JAK2 Gene
Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia  Christopher.

Allele-Specific Polymerase Chain Reaction for the Imatinib-Resistant KIT D816V and D816F Mutations in Mastocytosis and Acute Myelogenous Leukemia  Christopher.
A Rapid Polymerase Chain Reaction-Based Screening Method for Identification of All Expanded Alleles of the Fragile X (FMR1) Gene in Newborn and High-Risk.

A Rapid Polymerase Chain Reaction-Based Screening Method for Identification of All Expanded Alleles of the Fragile X (FMR1) Gene in Newborn and High-Risk.
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.

Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Screening for Mutations in Kidney-Related Genes Using SURVEYOR Nuclease for Cleavage at Heteroduplex Mismatches  Konstantinos Voskarides, Constantinos.

Screening for Mutations in Kidney-Related Genes Using SURVEYOR Nuclease for Cleavage at Heteroduplex Mismatches  Konstantinos Voskarides, Constantinos.
Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J

Jonathan A. Schumacher, Stephen D. Jenson, Kojo S. J
Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences  Marco Musso, Renata Bocciardi, Sara Parodi,

Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences  Marco Musso, Renata Bocciardi, Sara Parodi,
Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G

Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G
Detection of Aberrant TERT Promoter Methylation by Combined Bisulfite Restriction Enzyme Analysis for Cancer Diagnosis  Seungjae Lee, Sumit Borah, Armita.

Detection of Aberrant TERT Promoter Methylation by Combined Bisulfite Restriction Enzyme Analysis for Cancer Diagnosis  Seungjae Lee, Sumit Borah, Armita.
Philippe Szankasi, Mohamed Jama, David W. Bahler 

Philippe Szankasi, Mohamed Jama, David W. Bahler 
Ming-Tseh Lin, Roy G. Rich, Royce F. Shipley, Michael J

Ming-Tseh Lin, Roy G. Rich, Royce F. Shipley, Michael J
Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.

Homogeneous Polymerase Chain Reaction Nucleobase Quenching Assay to Detect the 1-kbp Deletion in CLN3 That Causes Batten Disease  Paul G. Rothberg, Denia.

Similar presentations

Ads by Google