1. Biosynthesis and Usage of Lysine
Group 4:
資工系博士班 彭千華
資工系博士班 彭士齊
資工系博士班 徐婕婷
資工系博士班 張家銘

2. Introduction of Lysine
Lysine (C6H14N2O2 )
~ an essential basic amino acid
~ net positive charge
~ commonly found on the surfaces of proteins and enzymes,
and sometimes appears in the active site.

3. Finding a pathway of Lysine

4. Finding a pathway of Lysine

5. Finding a pathway of Lysine

6. Finding a pathway of Lysine

7. Graph of Glycolysis

8. Graph of TCA cycle

9. Graph of Aspartate Metabolism

10. Graph of Lysine Biosynthesis

11. Biosynthesis of Threonine and Methionine

16. al

19. R39
R30
R31
R32
R33
R34
R35
R36
R37
R38

20. R41
R40
R39

21. R42
R43
R44

22. max R38
subject to
R1=100
R1-R2=0
R2-R3=0
R3-R4=0
R4-R5=0
R5-R6=0
R6-R7=0
R7-R8=0
R8-R9-R16=0
R9-R10=0
R10-R11=0
R11-R12=0
R12-R13-R17=0
R13-R14=0
R14-R15=0
R16+R28-R29=0
R17-R18=0
R18-R19=0
R19-R20=0
R20-R21=0
R21-R22=0
R22-R23=0
R23-R24=0
R24-R25=0
R25-R26=0
R26-R27=0
R27-R28=0
R29-R30=0
R30-R31=0
R31-R39-R32=0
R32-R33=0
R33-R34=0
R34-R35=0
R35-R36=0
R36-R37=0
R37-R38=0
R40-R41=0
R39-R40-R42=0
R42-R43=0
R43-R44=0
-100<=Ri<=100

23. Simplex Method

35. Results Input data: E.coli reactions (KEGG) Result1 Result2 :
Wild-type (bound: -100~100, glucose=100)
Methionine=0, Threonine=0, Lysine=47.5
Result2 :
Mutant (Methionine=0, Threonine=0, glucose=100)
Methionine=0, Threonine=0, Lysine=0

37. Thank You!!