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13.2 – Manipulating DNA
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The Tools of Molecular Biology
Genetic engineering – making changes in the DNA code of a living organism DNA extraction – by a simple chemical procedure Cutting DNA – most DNA to large to be analyzed Can be cut precisely at restriction enzymes Separating DNA – gel electrophoresis
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Using the DNA Sequence After the DNA is shortened up it can now be read, studied and even changed The function of different genes can now be discovered
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Gel Electrophoresis DNA plus restriction enzyme Power source
Longer fragments Shorter fragments Mixture of DNA fragments Gel
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