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Published byΔιοκλῆς Βουρδουμπάς Modified over 5 years ago
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Differential proteolytic activity and induction of apoptosis in fibrous versus atheromatous plaques in carotid atherosclerotic disease Theresa Jacob, PhD, Enrico Ascher, MD, Anil Hingorani, MD, Yevgeniy Khandros, Boris Tsemekhin, MD, Linda Zeien, MD, Yilmaz Gunduz, MD Journal of Vascular Surgery Volume 33, Issue 3, Pages (March 2001) DOI: /mva Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions
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Fig. 1 Apoptosis in fibrous and atheromatous plaque. Yellow-green fluorescence represents TUNEL-positive apoptotic bodies against red background of propidium iodide counterstained nuclei of nonapoptotic cells. (Original magnification ×1000.) Journal of Vascular Surgery , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions
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Fig. 2 Apoptosis in fibrous and atheromatous plaque. Differences in incidence of apoptosis between fibrous and atheromatous plaque. Cumulative data of all atheromatous and fibrous plaques were obtained. Number of apoptotic cells per thousand cells were counted manually at high-power magnification (original magnification ×1000) for each specimen (random fields were selected). Journal of Vascular Surgery , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions
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Fig. 3 Immunohistochemical localization of CPP-32 in atheromatous plaques. Note positive staining (brown) for CPP-32. (Original magnification ×400.) B, Immunohistochemical localization of Fas in atheromatous plaques. Note positive staining result (brown) for Fas. (Original magnification ×400.) C, Negative control. (Original magnification ×400.) Journal of Vascular Surgery , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions
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Fig. 4 Gelatin zymography of atheromatous and fibrous plaque extracts. (Representative zymogram with one atheromatous specimen extract and one fibrous specimen extract. A total of five atheromatous plaques and five fibrous plaques were analyzed.) Note prominent bands of gelatinolytic activity in atheromatous plaque. Molecular weights are shown on the left. C, Positive control; Ath, atheromatous; Fib, fibrous. Activities ranged from 68 to 94 kDa. Journal of Vascular Surgery , DOI: ( /mva ) Copyright © 2001 Society for Vascular Surgery and The American Association for Vascular Surgery Terms and Conditions
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