1. miR-210 is not involved in systemic hypoxia/TOR signalling.
miR-210 is not involved in systemic hypoxia/TOR signalling. (A) miR-210 expression was not induced in fly heads by 2.5% oxygen for 6 h, in contrast to the hypoxia-induced Scylla gene (**P > 0.01, t test, n = 3). (B) miR-210 expression was abolished in miR-210Δ mutants as shown by qRT-PCR on fly heads (****P < ; t test, n = 3). (C)miR-210Δ mutants developed normally under 10% oxygen, in contrast to Sima (HIF-1α) mutants (***P < 0.001; one-way ANOVA, n = 250). (D)miR-210Δ mutants survived 14 h of 1% oxygen, in contrast to heterozygous Sima (HIF-1α) mutants (***P < 0.001; one-way ANOVA, n = 50). (E, F) Lifespan (E) and starvation response (F) of miR-210Δ females was similar to wild-type flies (log-rank test, n = 100–200) and the lifespan, but not the starvation resistance, of miR-210Δ males was slightly extended (**P < 0.01, log rank test, n = 200). (G) miR-210Δ had a slightly increased weight compared with control flies (*P < 0.05; t test, n ∼ 50). (H)miR-210Δ mutants showed no difference in fecundity (t test, n = 100).
Carina M Weigelt et al. LSA 2019;2:e
© 2019 Weigelt et al.