1. WDR11 is required for ciliogenesis
WDR11 is required for ciliogenesis Immunofluorescence images of MEFs derived from E12.5 WT and null embryos. Arl13b stains axoneme and gamma‐tubulin (g‐TUB) stains the basal bodies of cilia. The percentages of ciliated cells were assessed against the total DAPI‐stained cells in random fields. The length of cilia was assessed in 150 cells per genotype by measuring the maximum projection using ImageJ. Data represent mean ± SEM from three independent experiments using two‐tailed unpaired t‐test (*****P < ). Scale bar, 50 μm.Choroid plexus sections of 8‐week‐old brain exhibited morphological alterations and lack of ciliary acetylated tubulin (ACT) staining in Wdr11−/−. Scale bars, 1 mm (main image) and 500 μm (zoomed images). TEM micrographs of longitudinal (1,000×) and horizontal (15,000×) sections of the choroid plexus epithelium show no significant difference in the 9+2 microtubule arrangement of the axoneme.Immunofluorescence analyses of NIH3T3 cells transfected with GFP‐tagged WDR11 construct and treated with purmorphamine (Pur), cyclopamine (Cyc), leptomycin B (Lep) and the solvent dimethylformamide (Solv) for 10 h before staining with anti‐ACT or anti‐g‐TUB antibodies followed by DAPI. Scale bar, 10 μm.The percentage of cells showing either nuclear or cytoplasmic localization of WDR11‐GFP were quantified in HEK293 cells after each treatment as in (C). Data from four independent experiments, counting 300–400 cells in each experiment, are presented as mean ± SEM with two‐way ANOVA followed by Bonferroni's post hoc test (****P < ).
Yeon‐Joo Kim et al. EMBO Rep. 2018;19:
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