1. CD11b+ alveolar macrophages in idiopathic pulmonary fibrosis (IPF) develop tube-like structures in vitro.
CD11b+ alveolar macrophages in idiopathic pulmonary fibrosis (IPF) develop tube-like structures in vitro. CD11b+ alveolar macrophages were cultured in Matrigel® for up to 31 days and inspected under white light or after fluorescent-labelling of cytoplasm (CellTracker™ Orange CMTMR; Invitrogen, Carlsbad, CA, USA) and nuclei (Hoechst stain; Invitrogen). Large tube-like structures (150 μm in diameter) were observed when cells from subjects with IPF were cultured in Matrigel® (BD Biosciences, San Jose, CA, USA) for 30 days. A series of confocal images were reconstructed in three-dimensional renderings and representative snapshots of tubular structures are presented as overlaid fluorescence a, b) with or c) without differential influence contrast.
Connie G. Glasgow et al. Eur Respir Rev 2012;21:
©2012 by European Respiratory Society