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Volume 65, Issue 4, Pages (April 2004)

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1 Volume 65, Issue 4, Pages 1467-1472 (April 2004)
Significance of the tissue kallikrein promoter and transforming growth factor-β1 polymorphisms with renal progression in children with vesicoureteral reflux  Guey-Jen Lee-Chen, Kuo-Pao Liu, Yi-Chun Lai, Huei-Shiuan Juang, Shu-Yi Huang, Ching-Yuang Lin  Kidney International  Volume 65, Issue 4, Pages (April 2004) DOI: /j x Copyright © 2004 International Society of Nephrology Terms and Conditions

2 Figure 1 Genotype analysis of human tissue kallikrein gene promoter polymorphism. Map of the oligonucleotide primers for polymerase chain reaction (PCR) (A). The promoter region is represented by gray rectangle and the position of the polymorphic region (-121 ∼-130) is shaded. Location of primers hKPM6, hKPMA, hKPMB, and hKPM7 is indicated with arrowheads. Electrophoresis of fluorescenced PCR products in linear polyacrylamide gels on an automated sequencer (B). Electrophoresis of danatured PCR products for SSCP and heteroduplex analyses (C). Electrophoresis of B allele-specific PCR amplified products in 2.2% agarose gel (D). The template genotypes in (B-D) are indicated by AH, BB, AK, AB, AA, BK, AH, HH, BH, and HK. Kidney International  , DOI: ( /j x) Copyright © 2004 International Society of Nephrology Terms and Conditions

3 Figure 2 Restriction analysis of transforming growth factor-β1 (TGF-β1) –509 T-C polymorphism. The amplified 808-bp fragments were digested with Bsu36I and separated in a 1.4% agarose gel. Lane M (HinfI digest of pGEM4 DNA) is used for size markers. The template genotypes are indicated by TC, TT, and CC. Kidney International  , DOI: ( /j x) Copyright © 2004 International Society of Nephrology Terms and Conditions

4 Figure 3 Renal survival curves in vesicoureteral reflux (VUR) children with progression to chronic renal insufficiency (CRI) by KLK1 gene promoter polymorphism. Kidney International  , DOI: ( /j x) Copyright © 2004 International Society of Nephrology Terms and Conditions

5 Figure 4 Renal survival curves in vesicoureteral reflux (VUR) children with progression to chronic renal insufficiency (CRI) by transforming growth factor-β1 (TGF-β1) gene promoter polymorphism. Kidney International  , DOI: ( /j x) Copyright © 2004 International Society of Nephrology Terms and Conditions

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