1. Darcy Permeability of Agarose-Glycosaminoglycan Gels Analyzed Using Fiber-Mixture and Donnan Models 
Kristin J. Mattern, Chalida Nakornchai, William M. Deen 
Biophysical Journal 
Volume 95, Issue 2, Pages (July 2008)
DOI: /biophysj
Copyright © 2008 The Biophysical Society Terms and Conditions

2. Figure 1
Elements of the microstructural model for the hydraulic permeability of an agarose-GAG membrane. Included were: a periodic, charged fiber model for GAG; a random, neutral fiber model for agarose; a mixing rule (applied twice) to average the permeabilities of differing fiber orientations or types; and a representation of agarose heterogeneity, as fiber clumps within a less dense matrix.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

3. Figure 2
Darcy permeability (κ) of agarose-GAG gels for a range of ionic strengths and GAG contents. The data are for 3vol/vol % agarose and three GAG levels: agarose without GAG (●); 54mg GAG/g agarose (▴); and 129mg GAG/g agarose (■). Error bars are one standard deviation for n=6. The predictions of the microstructural and macroscopic (Donnan) models are shown by dashed and solid curves, respectively. The overall volume fractions corresponding to the three GAG contents are ϕGAG=0, , and , respectively. The parameters used to describe gel heterogeneity were ɛ=0.1 and ϕm/ϕs=0.020.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

4. Figure 3
Schematics of models for gels with regions of higher fiber density (shaded) and lower fiber density (open): (a) layers in series, (b) layers in parallel, (c) denser clumps, and (d) less dense voids.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

5. Figure 4
Effective Darcy permeability of a two-region composite (κeff) compared to that for a homogeneous system with the same total fiber volume fraction (κhom). Permeabilities for various models are shown as a function of the ratio of the solid volume fractions in the two regions (ϕm/ϕs). In each case ϕ=0.03 (overall volume fraction of solid) and ɛ=0.1 (fraction of total volume occupied by region s). The results for spherical inclusions (clumps or voids), parallel regions, and series regions are based on Eqs. 5, 11, and 12, respectively; those for a homogeneous material are from Eq. 3.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

6. Figure 5
Darcy permeability (κ) of agarose-dextran gels. The results are for 4vol/vol % agarose (■,□) or 8vol/vol % agarose (▴▵,), the open symbols representing data from Kosto and Deen (8) and the solid symbols data from White and Deen (7). (The results from the latter study are for 500kDa dextran, with values of ϕdex corrected by using the binding efficiencies measured in the former study.) The curves are predictions obtained from a fiber-mixture model with ra=1.6nm, rdex=0.33nm, ɛ=0.1, and ϕm/ϕs=0.11 and 0.14 for 4vol/vol % and 8vol/vol % agarose, respectively. The heterogeneity parameters (ɛ and ϕm/ϕs) were chosen to fit the data of Kosto and Deen (8) for pure agarose.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions

7. Figure 6
Darcy permeability (κ) of agarose-GAG gels, compared to predictions of the macroscopic model. The data are as in Fig. 2, and the curves are now best-fit results obtained by adjusting the fixed-charge concentrations and neutral permeability. The best-fit charge concentrations were Cm=5.2meq/L for 54mg/g and Cm=8.8meq/L for 129mg/g. In calculations for GAG-containing gels, the permeability of the uncharged system (κ0) was equated to the measured value at C0=1M.
Biophysical Journal  , DOI: ( /biophysj )
Copyright © 2008 The Biophysical Society Terms and Conditions