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Expression of stem cell factor and its receptor in human fetal and adult ovaries
Ronit Abir, Ph.D., Benjamin Fisch, M.D., Ph.D., Shaoguang Jin, M.D., Mirian Barnnet, Gania Kessler-Icekson, Ph.D., Asangla Ao, Ph.D. Fertility and Sterility Volume 82, Pages (October 2004) DOI: /j.fertnstert Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Immunocytochemical photographs of stem cell factor (SCF) expression. (A) Section of a human ovary from a fetus of 33 gestational weeks (GW) with achondroplasia. Note the primary follicle and the brown staining in its oocyte indicating SCF expression. (Magnification ×400.) (B) Section of human ovary from a 14-year-old girl with osteosarcoma. Note the primordial follicles and the brown staining in the oocytes indicating SCF expression. (Magnification ×400.) (C) Negative control of a human ovary from a 27-GW-old fetus with cardiac and pulmonary malformations. Note the primordial follicles, the overall purple-blue staining, and the lack of brown staining. (Magnification ×400.) Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions
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FIGURE 2 Immunocytochemical photographs of stem cell factor receptor (SCF-R) expression. (A) Section of a human ovary from a fetus of 33 gestational weeks (GW) with achondroplasia. Note the primordial follicles and the brown staining in the oocytes, indicating SCF-R expression. (Magnification ×400.) (B) Section of human ovary from a 14-year-old girl with osteosarcoma. Note the primary follicle and the brown staining in its oocyte indicating SCF-R expression. (Magnification ×400.) (C) Negative control of an ovary from 38-year-old woman who had an ovarian cyst. Note the primary follicle, the overall purple-blue staining, and the lack of brown staining. (Magnification ×400.) Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions
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FIGURE 3 Gel electrophoresis of RT-PCR product for stem cell factor (SCF), stem cell factor receptor (SCF-R), and hypoxanthine phosphoribosyl transferase (HPRT) from human adult and fetal ovaries. Lanes 2, 3 and 6: adult human ovaries. Lanes 4, 5, 7, 8: fetal ovaries. W: Women; F: Fetus; M: pBR322 Hae III digest DNA on polyacrylamide gel (A to D). A: SCF RT-PCR product 193 bp. B: SCF-R 196 bp. C: HPRT 226 bp. D: RT negative controls. GW, gestational weeks; yrs, years. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2004 American Society for Reproductive Medicine Terms and Conditions
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