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Volume 118, Issue 3, Pages (March 2000)

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1 Volume 118, Issue 3, Pages 582-590 (March 2000)
CD4+ T cells play an important role in acute experimental pancreatitis in mice  Anne Demols*,‡, Olivier Le Moine*,‡, Fabrice Desalle§, Eric Quertinmont‡, Jean-Luc van Laethem*,‡, Jacques Devière*,‡  Gastroenterology  Volume 118, Issue 3, Pages (March 2000) DOI: /S (00) Copyright © 2000 American Gastroenterological Association Terms and Conditions

2 Fig. 1 Immunohistochemical staining of T lymphocytes. High-power view (200×) showing positive staining (dark brown, red arrow) for CD4+ and CD8+ T cells. T cells are invading the pancreas from blood vessels and are present around bordering acini. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

3 Fig. 2 Amylase and lipase serum levels (U/L) in BALB/c (2) and nude mice (■) before (T0) and 3 (T3) and 6 (T6) hours after start of cerulein challenge (n = 8 in each group). Results are expressed as means ± SEM. *P < 0.05. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

4 Fig. 3 RT-PCR detection of intrapancreatic IL-12 and IFN-γ (and internal standard β-actin) in wild-type (BALB/c), nude, and T-transferred nude (T Nude) mice 6 hours (T6) after start of cerulein injections. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

5 Fig. 4 RT-PCR detection of intrapancreatic chemokines (and internal standard β-actin) in wild-type (BALB/c) and nude mice at baseline (T0) and at T6. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

6 Fig. 5 Amylase and lipase serum levels (U/L) in wild-type (BALB/c), nude, and T-transferred nude (T Nude) mice at baseline (2) and T6 (■). Results represent values obtained from 3 independent experiments totaling 8 mice in each group. *P < 0.05, T nude compared with nude mice. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

7 Fig. 6 Examples of flow-cytometric analysis assessing CD4+ and CD8+ T-cell subsets on (A) splenocytes of control mouse (CD4+, 47.7%; CD8+, 16.1%), (B) CD4+ T cell–depleted mouse (CD4+, 0%; CD8+, 24.1%), and (C) CD8+ T cell–depleted mouse (CD4+, 36.8%; CD8+, 0.4%) 6 hours after start of cerulein challenge. Analysis was performed on 5000 gated cells, and results are expressed as percentage of 5000 gated cells. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

8 Fig. 6 Examples of flow-cytometric analysis assessing CD4+ and CD8+ T-cell subsets on (A) splenocytes of control mouse (CD4+, 47.7%; CD8+, 16.1%), (B) CD4+ T cell–depleted mouse (CD4+, 0%; CD8+, 24.1%), and (C) CD8+ T cell–depleted mouse (CD4+, 36.8%; CD8+, 0.4%) 6 hours after start of cerulein challenge. Analysis was performed on 5000 gated cells, and results are expressed as percentage of 5000 gated cells. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

9 Fig. 6 Examples of flow-cytometric analysis assessing CD4+ and CD8+ T-cell subsets on (A) splenocytes of control mouse (CD4+, 47.7%; CD8+, 16.1%), (B) CD4+ T cell–depleted mouse (CD4+, 0%; CD8+, 24.1%), and (C) CD8+ T cell–depleted mouse (CD4+, 36.8%; CD8+, 0.4%) 6 hours after start of cerulein challenge. Analysis was performed on 5000 gated cells, and results are expressed as percentage of 5000 gated cells. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

10 Fig. 7 Amylase and lipase serum levels (U/L) in BALB/c (2) and CD4+ T cell–depleted BALB/c (■) mice (n = 5 in each group) at baseline and 6 hours after cerulein injections. Results are expressed as means ± SEM. *P < 0.05. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

11 Fig. 8 RT-PCR detection of intrapancreatic Fas ligand (and internal standard β-actin) in wild-type (BALB/c), nude, and T nude mice 6 hours after start of cerulein challenge. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

12 Fig. 9 Amylase and lipase serum levels (U/L) in wild-type C57BL/6 (2) and GLD (■) mice 6 hours after start of cerulein challenge (n = 5 in each group). Results are expressed as means ± SEM. *P < 0.05. Gastroenterology  , DOI: ( /S (00) ) Copyright © 2000 American Gastroenterological Association Terms and Conditions

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