1. Database for Microarray data management
분자유전체학과
이영애

2. Purpose Database Analysis of Microarray data Selection of target genes
Target genes information
-Accession number
-Gene Name
-Ratio
-Functional family
-Function: OMIM
-Transcript sequence
-RT-PCR : Primer sequence
Annealing temperature
Ratio
Functional study

3. Analysis of the ultraviolet B response in primary human
PNAS, 2002
Analysis of the ultraviolet B response in primary human
keratinocytes using oligonucleotide microarrays
Angela Sesto

4. Materials and Methods UVB (10, 20, 40mJ/cm2)
Primary human Keratinocytes
After 0, 4, 24 hr, samples
6,000 transcripts; Affymetrix GeneChip HuGeneFL arrays

5. Analysis of microarray data
GENECHIP analysis software (Affymetrix):
539 regulated transcripts (~10 %)

6. - Cluster analysis: GENECLUSTER 1.1
Doses: , 10 mJ/cm2; , 20 mJ/cm2; , 40 mJ/cm2

7. - Biological functions: GENECARDS database

8. Contents for Database Target genes information
1) Accession number:
2) Gene Name:
3) Ratio of change
4) Functional family: GENECARDS database
5) Function: OMIM,
6) Transcript sequence:
7) RT-PCR
- Primer sequences: (Electronic PCR)
- Annealing temperature
- Ratio