1. Transport of Biotin in Human Keratinocytes
Franziska Grafe, Matthias Brandsch 
Journal of Investigative Dermatology 
Volume 120, Issue 3, Pages (March 2003)
DOI: /j x
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Time course of [3H]biotin uptake in HaCaT cells. Uptake of [3H]biotin (6nM) was measured in buffer (pH 7.5) containing 140mM sodium chloride or choline chloride. Data are shown as mean±SEM, n=3.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Effect of sodium and chloride gradients on specific [3H]biotin uptake in HaCaT cells. Uptake of [3H]biotin (6nM) was measured in buffer (pH 7.5) with or without sodium or chloride gradients and in the presence or absence of 10mM unlabeled biotin for 5min. Data are shown as mean±SEM, n=3.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Saturation kinetics of biotin uptake in the absence or presence of pantothenic acid into HaCaT cells. Confluent monolayers were incubated in buffer (pH 7.5) with [3H]biotin (12nM) for 5min containing increasing concentrations of biotin (1–50μM) with or without (control) pantothenic acid (2μM). The results represent specific (saturable) uptake values after subtraction of the nonmediated component of transport ([3H]biotin uptake in the presence of 10mM unlabeled biotin). The results are shown as mean±SEM, n=3–13. Inset: Eadie-Hofstee transformation of the data.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Substrate specificity of biotin uptake in HaCaT cells. Uptake of [3H]biotin (6nM) was measured in the absence (control) or presence of increasing concentrations of various related unlabeled substances at pH 7.5 for 5min. The results are shown as mean ± SEM, n=3–8.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Effect of time and sodium ions on the very high affinity biotin transport in HaCaT cells. Uptake of [3H]biotin (0.5nM) was measured in buffer (pH 7.5) containing 140mM NaCl for 30min. Inset: 30min uptake in the presence of 140mM sodium chloride or 140mM choline chloride. Data are shown as mean±SEM, n=3–6.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

7. Figure 6
Inhibition of the very high affinity biotin transport in HaCaT cells. Confluent monolayers were incubated in buffer (pH 7.5) with [3H]biotin (1nM) for 30min with or without inhibitors. The results are shown as mean ± SEM, n=3–5.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions

8. Figure 7
Saturation kinetics of the very high affinity biotin uptake in HaCaT cells. Uptake was measured at increasing concentrations of 3H-labeled biotin in the range 0.1–10nM at pH 7.5. The results represent specific (saturable) uptake rates after subtraction of the nonmediated component of transport, which was calculated by nonlinear regression of total uptake rates (nonsaturable transport constant kd=12.3±0.5μl per 30min per mg protein). The results are shown as mean ± SEM, n=4. Inset: Eadie-Hofstee transformation of the data.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 2003 The Society for Investigative Dermatology, Inc Terms and Conditions