1. Volume 7, Issue 9, Pages 1497-1500 (September 2014)
The Rice Restorer Rf4 for Wild-Abortive Cytoplasmic Male Sterility Encodes a Mitochondrial-Localized PPR Protein that Functions in Reduction of WA352 Transcripts 
Huiwu Tang, Dangping Luo, Degui Zhou, Qunyu Zhang, Dongsheng Tian, Xingmei Zheng, Letian Chen, Yao-Guang Liu 
Molecular Plant 
Volume 7, Issue 9, Pages (September 2014)
DOI: /mp/ssu047
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Cloning and Functional Analysis of the Restorer Gene Rf4 for CMS-WA.
(A) Genetic and physical mappings of Rf4. Numbers of the markers also indicate the approximate positions (kb) on chromosome 10. PPR4(Komori at al., 2004; Wang et al., 2006) and PPR8 (this study) are absent in japonica cultivars. N, ZH, M, I, Z, J, 93 indicate alleles of these genes in the cultivars Nip, ZH11, MH63, IR24, ZS97A, J23A, and 93–11, respectively. Small triangles indicate two insertions (1476bp and 105bp) in the indica PPR9-409 alleles.
(B) Morphology of anthers and pollen grains of two T1 plants (T1-1, T1-2) carrying WA-cytoplasm and the hemizygous PPR9-782-M transgene, and a plant (T1-1S) without the transgene segregated from the T1 generation, which is equivalent to J23A. Pollen grains were stained with 1% I2-KI.
(C) Seed-setting rates of the male fertility-restored plants of T1-1 and T1-2 lines.
(D) Two fusion proteins, PPR9-782-M:eGFP and MTS:mOrange, were co-expressed transiently in a rice protoplast cell. The mitochondrial transit sequence (MTS) was derived from Rf1b; mOrange, a fluorescent protein variant. Scale bars = 10 μm.
(E, F)WA352 expression in young panicles of J23A, ZSRf4, and PPR9-782-M T1 plants (T1-1, T1-2) was analyzed by RT–PCR (D) and qRT–PCR (E). The mitochondrial atp6 was used as an internal control. Data shown (E) are Mean ± SD.
Molecular Plant 2014 7, DOI: ( /mp/ssu047)
Copyright © 2014 The Authors. All rights reserved. Terms and Conditions