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MY PLAN: Acquire reads Analyze reads Lengthen reads Find open reading frames Find proteins.

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Presentation on theme: "MY PLAN: Acquire reads Analyze reads Lengthen reads Find open reading frames Find proteins."— Presentation transcript:

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5 MY PLAN: Acquire reads Analyze reads Lengthen reads
Find open reading frames Find proteins

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7 Analyze Massive similarity of first 100 nt Need for editing

8 Lengthening read

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13 Finding overlap within Making sure to always extend the ORF

14 Contig4-1 Length: 2751 nt ORF: 77-601 795-2108 2370-2702
Made up of 6 different reads

15 Open Reading Frame No hits compared to known viruses
Only matches amino acids 8-126

16 Open Reading Frame No hits compared to known viruses
Only matches amino acids 8-126

17 All Archea, Euryarchaeota
Thermococcus sp. AM4 hyperthermophilic archaeon Thermococcus is extremely abundant in vent environments and this particular species and strain has the amazing ability to get its carbon and energy from carbon monoxide oxidation, resulting in hydrogen production. Iorn-sulfur protein

18 All Archea, Euryarchaeota
Methanocaldococcus jannaschii DSM 2661 Methane-producing thermophilic archeon. This organism is an obligately anaerobic methane-producing archeon and was the first representative of the archaeal domain to be completely sequenced. Hypothetical protein

19 All Archea, Euryarchaeota
Aciduliprofundum boonei T469 Widely distributed at deep-sea vents- only information has been in clone libraries. This is the first isolate, and it's sister lineage are the Thermplasmales Phosphoadenosine phosphosulfate reductase family protein reduction of PAPS to phospho-adenosine- phosphate (PAP)

20 Bacillus thuringiensis str. Al Hakam
This organism, also known as BT, is famous for the production of an insecticidal toxin. This organism, like many other Bacilli, is found in the soil, integrase Integrase is an enzyme produced by a retrovirus that enables its genetic material to be integrated into the DNA of the infected cell.


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