1. Association of the Gene Encoding Wingless-Type Mammary Tumor Virus Integration- Site Family Member 5B (WNT5B) with Type 2 Diabetes 
Akio Kanazawa, Syuuichi Tsukada, Akihiro Sekine, Tatsuhiko Tsunoda, Atsushi Takahashi, Atsunori Kashiwagi, Yasushi Tanaka, Tetsuya Babazono, Masafumi Matsuda, Kohei Kaku, Yasuhiko Iwamoto, Ryuzo Kawamori, Ryuichi Kikkawa, Yusuke Nakamura, Shiro Maeda 
The American Journal of Human Genetics 
Volume 75, Issue 5, Pages (November 2004)
DOI: /425340
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

2. Figure 1
LD mapping around the WNT5B gene. LD coefficients (D′ and Δ) between every two SNPs around the SNP in intron 4 (+438, C/G) were calculated. Minor allele frequencies of all SNPs used in this analysis are >10%. An asterisk (*) highlights the SNP in intron 4 (+438, C/G).
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

3. Figure 2
Polymorphisms identified in the WNT5B gene. The number sign (#) indicates the SNP in intron 4 (+438, C/G) that showed strong association. An asterisk (*) indicates SNPs selected from the IMS-JST Japanese SNP database.
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

4. Figure 3
A, Analysis of haplotype structure and estimated haplotype frequencies within the WNT5B gene. 56 = intron 3+439; 57 = intron ; 58 = intron 4+438; 67 = intron ; 71 = intron a, Haplotype 1/others: χ2=4.41; P=.036; OR 1.24, 95% CI 1.01–1.52. b, Haplotype 2/others: χ2=5.59; P=.018, OR .77, 95% CI .62–.96. B, Plots of LD coefficients (Δ) between the SNP at intron and other SNPs within the WNT5B gene.
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

5. Figure 4
Expression pattern of the Wnt5b gene in 3T3-L1 cells during differentiation. Quantitative real-time PCR was used to evaluate the amount of Wnt5b mRNA. Total RNA was extracted from 3T3-L1 cells at 0, 1, 2, 3, 4, 5, 6, 7, and 10 d after induction of differentiation.
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

6. Figure 5
Effects of Wnt5b gene overexpression in 3T3-L1 cells on adipogenesis. An adenovirus vector encoding the Wnt5b gene was infected into 3T3-L1 cells 48 h before induction of differentiation at a multiplicity of infection of 50. A, Lipid droplets stained with oil red O at 5 d after induction of differentiation. B, Quantitative analysis of oil red O staining. Values are mean±SE of five independent experiments. An asterisk (*) indicates P<.05 versus control cells (LacZ).
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions

7. Figure 6
Effects of Wnt5b gene overexpression in 3T3-L1 cells on adipocyte gene expression: the expression of Pparg (A), Cebpa (B), apM1 (C), and Lep (D) in Wnt5b overexpressing 3T3-L1 cells at 3 and 7 d after induction of differentiation. The results obtained from four independent experiments are presented. An asterisk (*) indicates that P<.05. A double asterisk (**) indicates P<.01 versus control cells (LacZ).
The American Journal of Human Genetics  , DOI: ( /425340)
Copyright © 2004 The American Society of Human Genetics Terms and Conditions