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Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice Dual‐recombinase fluorescence lineage.

Published byMarjatta Parviainen Modified over 5 years ago

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Presentation on theme: "Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice Dual‐recombinase fluorescence lineage."— Presentation transcript:

1 Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice Dual‐recombinase fluorescence lineage tracing of EMT in KPF (FSF‐KrasG12D/+;Trp53frt/+;Pdx1‐Flp);αSMA‐Cre;R26Dual mice AGenetic strategy to induce oncogenic KrasG12D and heterozygous p53 loss using the Pdx1‐Flp‐FRT recombination system in KPF mice of the dual‐recombinase system (DRS).BRepresentative sections of PanIN (stage 1‐3) and PDAC lesions of KPF mice stained by hematoxylin and eosin (H&E) and cytokeratin‐19 (CK19) immunohistochemistry. These panels are also depicted in Appendix Fig S3.CRepresentative H&E‐stained and CK19‐immunostained sections of liver metastases (arrows) in KPF mice. These panels are also depicted in Appendix Fig S3.DGenetic strategy to induce EGFP expression in Pdx1‐Flp lineage and tdTomato expression in αSMA‐Cre lineage (either myofibroblasts or αSMA‐expressing EMT cancer cells) using a novel Rosa26‐CAG‐loxP‐frt‐Stop‐frt‐FireflyLuc‐EGFP‐loxP‐RenillaLuc‐tdTomato (R26Dual) tracer. Yang Chen et al. EMBO Mol Med. 2018;emmm © as stated in the article, figure or figure legend

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