1. Histologic and ultrastructural evaluation of fresh and frozen-thawed human ovarian xenografts in nude mice 
Michelle Nisolle, M.D., Ph.D., Françoise Casanas-Roux, B.S., Jianping Qu, M.D., Ph.D., Pietro Motta, M.D., Ph.D., Jacques Donnez, M.D. 
Fertility and Sterility 
Volume 74, Issue 1, Pages (July 2000)
DOI: /S (00)

2. FIGURE 1
Subcutaneous frozen-thawed ovarian graft. (A), The ovarian graft is 2.03 mm long × 0.96 mm wide (stain, Gomori’s trichrome; original magnification, ×4). (B), Five primordial or primary follicles are seen at the periphery of the ovarian graft (stain, Gomori’s trichrome; original magnification, ×25).
Nisolle. Ovarian xenografts in nude mice. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

3. FIGURE 2
Intraperitoneal frozen-thawed ovarian graft. (A), Normal looking primary follicles are noted in the fibrosis area (stain, Gomori’s trichrome; original magnification, ×40). (B), A mitotic figure is observed in a secondary follicle (stain, Gomori’s trichrome, original magnification, ×40).
Nisolle. Ovarian xenografts in nude mice. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

4. FIGURE 3
Primordial follicle from frozen-thawed human ovarian biopsy. Flattened follicle cells (F) surround the ovocyte (O). The ovocyte organelles, among which are recognizable mitochondria (m) and multivesicular bodies (mvb), can be seen grouped near the nucleus (N). Ovocyte microvilli (mi) are seen projecting toward follicle cells. TEM, ×1,250.
Nisolle. Ovarian xenografts in nude mice. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )

5. FIGURE 4
Frozen-thawed human ovarian tissue implanted in mouse peritoneum. By TEM, the high cellularity of the implanted tissue seems, in part, due to the presence of numerous white blood cells (W) that engulf the capillaries and infiltrate the interstitial tissue. Ve, vessels and round structures (arrows) containing a largely vacuolated core. TEM, ×1,600.
Nisolle. Ovarian xenografts in nude mice. Fertil Steril 2000.
Fertility and Sterility  , DOI: ( /S (00) )