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Diesel exhaust particles exert acute effects on airway inflammation and function in murine allergen provocation models  Minqi Hao, MD, PhD, Stephania.

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Presentation on theme: "Diesel exhaust particles exert acute effects on airway inflammation and function in murine allergen provocation models  Minqi Hao, MD, PhD, Stephania."— Presentation transcript:

1 Diesel exhaust particles exert acute effects on airway inflammation and function in murine allergen provocation models  Minqi Hao, MD, PhD, Stephania Comier, PhD, Meiying Wang, MD, James J. Lee, PhD, Andre Nel, MD, PhD  Journal of Allergy and Clinical Immunology  Volume 112, Issue 5, Pages (November 2003) DOI: /j.jaci

2 FIG 1 Use of a mild sensitization protocol to show the effects of nebulizer DEPs on AHR and inflammation. A Outline of the protocol. B Total and differential BAL cell counts. C Serum OVA-specific antibody levels, as determined by means of ELISA. ∗P < .05, groups 3 and 4 versus group 2. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

3 FIG 1 Use of a mild sensitization protocol to show the effects of nebulizer DEPs on AHR and inflammation. A Outline of the protocol. B Total and differential BAL cell counts. C Serum OVA-specific antibody levels, as determined by means of ELISA. ∗P < .05, groups 3 and 4 versus group 2. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

4 FIG 1 Use of a mild sensitization protocol to show the effects of nebulizer DEPs on AHR and inflammation. A Outline of the protocol. B Total and differential BAL cell counts. C Serum OVA-specific antibody levels, as determined by means of ELISA. ∗P < .05, groups 3 and 4 versus group 2. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

5 FIG 4 The effects of nebulizer DEPs on IL-5 transgenic mice: A, protocol outline; B, DEP exposure leads to an increase in methacholine-induced AHR in IL-5 transgenic mice. ∗P < .05, DEPs versus saline at 100 mg/mL methacholine. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

6 FIG 4 The effects of nebulizer DEPs on IL-5 transgenic mice: A, protocol outline; B, DEP exposure leads to an increase in methacholine-induced AHR in IL-5 transgenic mice. ∗P < .05, DEPs versus saline at 100 mg/mL methacholine. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

7 FIG 6 Delayed DEP delivery induced AHR in the classical sensitization model. A, Protocol outline. B, Methacholine challenge and WBP. DEP effects in the postchallenge model have been confirmed in numerous repeat experiments. ∗P < .05, group 5 versus group 2 at 25 and 50 mg/mL methacholine. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

8 FIG 6 Delayed DEP delivery induced AHR in the classical sensitization model. A, Protocol outline. B, Methacholine challenge and WBP. DEP effects in the postchallenge model have been confirmed in numerous repeat experiments. ∗P < .05, group 5 versus group 2 at 25 and 50 mg/mL methacholine. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

9 FIG 2 DEP exposure leads to an increase in methacholine-induced AHR in the mild sensitization protocol. Penh was measured in a Buxco box, as previously described.17 ∗P < .05, group 3 versus group 4. Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

10 FIG 3 Histology to show airway inflammation and MBP and mucin staining in the mild sensitization protocol. A, Hematoxylin and eosin staining (a, group 1; b, group 2; c and e, group 3; d and f, group 4). B, MBP staining (a, group 3; b, group 4). C, Periodic acid-Schiff staining (a, group 1; b, group 2; c, group 3; d, group 4; original magnification 100×). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

11 FIG 3 Histology to show airway inflammation and MBP and mucin staining in the mild sensitization protocol. A, Hematoxylin and eosin staining (a, group 1; b, group 2; c and e, group 3; d and f, group 4). B, MBP staining (a, group 3; b, group 4). C, Periodic acid-Schiff staining (a, group 1; b, group 2; c, group 3; d, group 4; original magnification 100×). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

12 FIG 3 Histology to show airway inflammation and MBP and mucin staining in the mild sensitization protocol. A, Hematoxylin and eosin staining (a, group 1; b, group 2; c and e, group 3; d and f, group 4). B, MBP staining (a, group 3; b, group 4). C, Periodic acid-Schiff staining (a, group 1; b, group 2; c, group 3; d, group 4; original magnification 100×). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

13 FIG 5 Histology to show the effects of nebulizer DEPs on lung inflammation. A, Hematoxylin and eosin staining (a and b, saline exposure; c and d, DEP exposure). B, Periodic acid-Schiff staining (same panels as Fig 5, A). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

14 FIG 5 Histology to show the effects of nebulizer DEPs on lung inflammation. A, Hematoxylin and eosin staining (a and b, saline exposure; c and d, DEP exposure). B, Periodic acid-Schiff staining (same panels as Fig 5, A). Journal of Allergy and Clinical Immunology  , DOI: ( /j.jaci )

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