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Human chorionic gonadotropin and 17-β estradiol regulation of human oviductin/oviduct specific glycoprotein mRNA expression in vitro  Christine Briton-Jones,

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Presentation on theme: "Human chorionic gonadotropin and 17-β estradiol regulation of human oviductin/oviduct specific glycoprotein mRNA expression in vitro  Christine Briton-Jones,"— Presentation transcript:

1 Human chorionic gonadotropin and 17-β estradiol regulation of human oviductin/oviduct specific glycoprotein mRNA expression in vitro  Christine Briton-Jones, Ph.D., Ingrid Hung Lok, M.R.C.O.G., Tony Tak Yu Chiu, Ph.D., Lai Ping Cheung, M.R.C.O.G., Christopher Haines, M.D.  Fertility and Sterility  Volume 80, Pages (September 2003) DOI: /S (03)

2 FIGURE 1 Oviductin/β-actin mRNA expression after addition of hCG in individual subjects. Subjects 1–4 were collected in the luteal phase, subjects 5–7 were collected in the late follicular phase, and subjects 8–10 were collected in the early follicular phase. Briton-Jones. hCG and E2 regulation of human oviductin mRNA. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

3 FIGURE 2 (A) Oviductin/β-actin mRNA expression after addition of E2 in individual subjects. Subjects 1–4 were collected in the luteal phase, subjects 5–7 were collected in the late follicular phase, and subjects 8–10 were collected in the early follicular phase. (B) PRAB/β-actin mRNA expression after addition of E2 in individual subjects. Subjects 1–4 were collected in the luteal phase, subjects 5–7 were collected in the late follicular phase, and subjects 8–10 were collected in the early follicular phase. Briton-Jones. hCG and E2 regulation of human oviductin mRNA. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )

4 FIGURE 3 RT-PCR products for ER-α (148 bp), ER-β (241 bp), and β-actin (543 bp), mw = 50 bp ladder molecular weight marker (a) mRNA from oviduct mucosal cells preculture with ER-α primers. (b) mRNA from oviduct mucosal cells preculture with ER-β primers. (c) mRNA from oviduct mucosal cells after 6 days of culture with ER-α primers. (d) mRNA from oviduct mucosal cells after 12 days of culture with ER-α primers. (e) mRNA from whole oviduct with ER-β primers. (f) mRNA from granulosa cells as a positive control for the ER-β primers. A separate RT-PCR reaction was performed on the same mRNA samples with β-actin primers to ensure the integrity of each mRNA sample. Briton-Jones. hCG and E2 regulation of human oviductin mRNA. Fertil Steril 2003. Fertility and Sterility  , DOI: ( /S (03) )


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