1. Volume 117, Issue 6, Pages 1427-1432 (December 1999)
Decreased food intake and body weight in pancreatic polypeptide-overexpressing mice 
Naohiko Ueno, Akio Inui, Masako Iwamoto, Toshihiro Kaga, Akihiro Asakawa, Minoru Okita, Mineko Fujimiya, Yoshito Nakajima, Yasukazu Ohmoto, Masaharu Ohnaka, Yutaka Nakaya, Jun-Ichi Miyazaki, Masato Kasuga 
Gastroenterology 
Volume 117, Issue 6, Pages (December 1999)
DOI: /S (99)
Copyright © 1999 American Gastroenterological Association Terms and Conditions

2. Fig. 1
Photomicrographs of PP-immunoreactive cells in sections of the pancreas (islets) from (A) control and (B) PP transgenic mice. PP immunoreactivity was dramatically increased in the pancreatic islets of transgenic mice, where PP-positive cells were spread in the middle of the islets.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

3. Fig. 2
Absence of transgene expression in tissues other than the pancreas assessed by (A–F) immunohistochemistry or (G) Northern blot analysis and RT-PCR. (A) Hypothalamus (control), (B) hypothalamus (tg), (C) liver (tg), (D) small intestine (tg), (E) muscle (M. gastrocnemius, control), and (F) muscle (M. gastrocnemius, tg). The area enclosed by arrowheads in A and B is the paraventricular nucleus of the hypothalamus. Bars = 100 μm. (G) Northern blot analysis and RT-PCR suggested a low amount of expression of the transgene in various tissues such as muscle.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

4. Fig. 2
Absence of transgene expression in tissues other than the pancreas assessed by (A–F) immunohistochemistry or (G) Northern blot analysis and RT-PCR. (A) Hypothalamus (control), (B) hypothalamus (tg), (C) liver (tg), (D) small intestine (tg), (E) muscle (M. gastrocnemius, control), and (F) muscle (M. gastrocnemius, tg). The area enclosed by arrowheads in A and B is the paraventricular nucleus of the hypothalamus. Bars = 100 μm. (G) Northern blot analysis and RT-PCR suggested a low amount of expression of the transgene in various tissues such as muscle.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

5. Fig. 3
Growth curve of PP transgenic (●) and control (○) (A) male and (B) female mice on a normal chow diet. Each group contained 7–8 mice. PP transgenic mice gained less weight than control mice, a result that was especially evident in male mice. *P < 0.05; **P < 0.01.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

6. Fig. 4
Food intake of PP transgenic (■) and control (□) (A) male and (B) female mice on a normal chow diet. Each group contained 15 mice. PP transgenic mice had reduced food intake compared with control littermates, a result that was especially evident in male mice. *P < 0.05; **P < 0.01.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

7. Fig. 4
Food intake of PP transgenic (■) and control (□) (A) male and (B) female mice on a normal chow diet. Each group contained 15 mice. PP transgenic mice had reduced food intake compared with control littermates, a result that was especially evident in male mice. *P < 0.05; **P < 0.01.
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

8. Fig. 5
Rate of gastric emptying of a solid meal in PP transgenic (■) and control (□) mice. The mice had free access to chow for 1 hour, and gastric emptying was assessed 2, 3, and 4 hours after eating. Each group contained 7–8 male mice. PP transgenic mice had a slower gastric emptying rate than control littermates (*P < 0.05).
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions

9. Fig. 6
Food intake and body weight of (A and C) PP transgenic or (B and D) control mice receiving an intraperitoneal injection of 5 μL of anti-PP serum (●) or preimmune serum (○). Each group contained 5 male mice. The immunoneutralization reversed the anorexia and the decrease in body weight of PP transgenic but not control mice (*P < 0.05; **P < 0.01).
Gastroenterology  , DOI: ( /S (99) )
Copyright © 1999 American Gastroenterological Association Terms and Conditions