1. Standard (Sanger) sequencing
Random ddNTP termination.
Template
Primer
Label can be added to the:
Primer
ddNTP –or-
Incorporated dNTPs

2. 454 sequencing (images by Roche)
Sample Input and Fragmentation: Genomic DNA or BACs are fractionated into small, 300- to 800-basepair fragments
Library Preparation: Short adaptors (A and B) - specific for both the 3' and 5' ends - are added to each single stranded fragment.
One Fragment = One Bead: Each fragment of the single-stranded DNA library is immobilized individually onto beads in a water-in-oil mixture.
emPCR (Emulsion PCR) Amplification: Each unique fragment is amplified in parallel to several million per bead.
One Bead = One Read: The clonally amplified fragments are loaded onto a PicoTiterPlate device for sequencing. Only one bead per well.
Auto fluidics flows individual nucleotides in a fixed order across the hundreds of thousands of wells containing one bead each. Addition of a nucleotide results in a chemiluminescent signal.

3. Solexa sequencing I Series of images taken from www.illumina.com

4. Solexa sequencing II
“Solexa sequencing”

5. Solexa sequencing III
“Solexa sequencing”

6. Rubber sequencing (2010 estimate)

7. Data overload
link