1. Adaptamers
Target A
Target B
Adaptamer

2. Why develop adaptamers?
To offer a new way to target substrates to cells (adaptamer linking cell surface and substrate).
To create a tool for studying cell-cell interactions (adaptamer linking two cell surfaces).
To provide a foundation for engineered catalysts (adaptamers linking 2 proteins).

3. Thrombin-binding domain
Streptavidin-binding domain
Thrombin
Linking region
Streptavidin

4. Adaptamer testing Bead surface streptavidin adaptamer thrombin
anti-thrombin antibody
Bead surface

5. Adaptamer functionality
Adaptamer = A35
Adaptamer = A15
adaptamer
Control: no thrombin
streptavidin
Control: beads lack streptavidin
thrombin
Bead surface

6. Adaptamer quenching… + + Results: Displacement strand
Displacement strand added +
No displacement strand added
Background fluorescence