1. IL-10 signaling in dendritic cells attenuates anti-Leishmania major immunity without affecting protective memory responses 
Mathilde J.H. Girard-Madoux, Kordula Kautz-Neu, Beate Lorenz, Julia L. Ober-Blöbaum, Esther von Stebut, Björn E. Clausen 
Journal of Investigative Dermatology 
Volume 135, Issue 11, Pages (November 2015)
DOI: /jid
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Enhanced clearance of L. major without loss of memory response in DC-IL10R-/- mice. Animals on a resistant C57BL/6 background were inoculated intradermally with a low dose of infectious-stage L. major clone VI (MHOM/IL/80/Friedlin) promastigotes (103 per ear). (a) Left panel: lesion volumes were measured weekly (longitudinally in one and the same mouse during the entire 16-week observation period) in three dimensions and are reported as ellipsoids ((a/2 × b/2 × c/2) × 4/3π). Mean±SEM of three combined experiments with n≥5 mice per group and time point is shown. Right panel: 6 months after the primary infection, mice were challenged with 103 infectious-stage L. major promastigotes per ear. ↓=Time point of reinfection (week 22). Mean±SEM of three combined experiments with n≥7 mice per group and time point is depicted. (b) Ear and spleen parasite numbers were determined at 6 weeks post infection using limiting dilution assays. (c) In weeks 16 and 22, numbers of parasites in ear lesions were quantified using limiting dilution assays. In b and c, mean parasite loads are shown as horizontal bars, and individual parasite counts/ear are shown as squares (n≥9 mice per group). *P<0.05 comparing non-TG and DC-IL10R-/- groups using Mann–Whitney test.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Enhanced Th1/Tc1 response in DC-IL10R-/- animals. Animals on a resistant C57BL/6 background were inoculated intradermally with a low dose of infectious-stage L. major promastigotes (103 per ear). (a and b) In week 6, lesion-draining submandibular LN cells were restimulated with soluble Leishmania antigen for 48 hours. Cytokine production was measured in the culture supernatants by Cytometric Bead Array according to the manufacturer’s instructions (BD, Heidelberg, Germany). Mean±SEM of three individually analyzed mice per group is presented. One representative out of n≥3 independent experiments is depicted. *P<0.05 and **P<0.01 comparing non-TG and DC-IL10R-/- using Student’s t-test. (c) In week 16 (after complete healing) and week 22 (before reinfection), antigen-specific cytokine release by LN cells from infected mice was determined by ELISA after restimulation with soluble Leishmania antigen (SLA). Mean±SEM of n≥9 individually analyzed mice per group is presented.
Journal of Investigative Dermatology  , DOI: ( /jid )
Copyright © 2015 The Society for Investigative Dermatology, Inc Terms and Conditions