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Hair Cycle-Specific Immunolocalization of Retinoic Acid Synthesizing Enzymes Aldh1a2 and Aldh1a3 Indicate Complex Regulation Helen B. Everts, Lloyd E. King, John P. Sundberg, David E. Ong Journal of Investigative Dermatology Volume 123, Issue 2, Pages (August 2004) DOI: /j X x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 1 Dot blot analysis of antibodies against recombinant whole proteins. Recombinant rat proteins Aldh1a2 (2) and Aldh1a3 (3) (0–25 pmol) were blotted onto nitrocellulose membranes, cut into strips, and exposed to affinity-purified antibodies against Aldh1a2 (a) or Aldh1a3 (b). Detection was by enhanced chemiluminescence. The image shows the two films for the two antibodies overlaying the strips of dot blotted protein. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 2 Aldh1a2 expression in adult mouse hair follicles. Immunohistochemical localization of Aldh1a2 in adult mouse hair follicles during telogen (a), anagen IIIa (b), anagen V (c), catagen V (d), and catagen VII (e). Bar=1 μm. Insets are higher magnifications of their respective follicles (arrows). Bar=0.1 μm all insets. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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Figure 3 Aldh1a3 expression in adult mouse hair follicles. Immunohistochemical localization of Aldh1a3 in adult mouse hair follicles during anagen IIIa (a), IIIa–b (b), IIIc (c), V (d), and early VI (e). Bar=1 μm. Insets are higher magnifications of their respective follicles (small arrows). Bar=0.1 μm all insets. Large arrows point to the sebaceous gland duct. Journal of Investigative Dermatology , DOI: ( /j X x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions
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