1. Xenotransplant cardiac chimera: immune tolerance of adult stem cells
Takayuki Saito, MD, PhD, Jin-Qiang Kuang, MD, Bindu Bittira, MD, Abdulaziz Al-Khaldi, MD, Ray C.-J Chiu, MD, PhD 
The Annals of Thoracic Surgery 
Volume 74, Issue 1, Pages (July 2002)
DOI: /S (02)

2. Fig 1
Experimental design. (LCA = left coronary artery; MSCs = marrow stromal cells.)
The Annals of Thoracic Surgery  , 19-24DOI: ( /S (02) )

3. Fig 2
Histochemical staining for β-galactosidase activity of mice marrow stromal cells in cultures. The transfected marrow stromal cells showed positive staining for β-galactosidase activity (blue color). (A) Culture-expanded mice marrow stromal cells before implantation. (B) Bone marrow specimen of the recipient rat to which mice marrow stromal cells were transplanted intravenously 13 weeks before. LacZ+ mice marrow stromal cells (arrow) were identified, whereas host marrow stromal cells were unstained. (C) Blood sample obtained at 1 day after ligating the left coronary artery in rats to which mice marrow stromal cells were transplanted intravenously 1 week before the ligation. LacZ+ mice marrow stromal cells (arrows) were also identified, whereas host cells were unstained. (Original magnification [A through C], ×100.)
The Annals of Thoracic Surgery  , 19-24DOI: ( /S (02) )

4. Fig 3
Histochemical staining for β-galactosidase activity of mice marrow stromal cells in the gross heart. Gross heart specimen of the recipient rat was harvested at 12 weeks after coronary artery ligation. Mice marrow stromal cells were transplanted intravenously 1 week before the ligation. After fixation, the heart was stained for β-galactosidase activity of mice marrow stromal cells. Bluish discoloration can be seen on the infarcted myocardium, whereas noninfarcted myocardium remains unstained. (A) Frontal view. (B) Lateral view.
The Annals of Thoracic Surgery  , 19-24DOI: ( /S (02) )

5. Fig 4
Histology and immunohistochemistry of the hearts with myocardial infarction. Mice marrow stromal cells were transplanted intravenously 1 week before coronary artery ligation. (A and B) Heart sample harvested at 8 weeks after ligation, and stained for β-galactosidase activity and hematoxylin and eosin. Numerous mice marrow stromal cells were identified mostly in the infarcted myocardium. (C) Heart sample harvested at 1 day after ligation. LacZ+ mice marrow stromal cells (arrows) were seen near vessel (asterisk) in an intact area. (D) Heart sample harvested at 1 day after ligation. LacZ+ cell (arrow) was seen in the interstitium of an intact myocardium. (E) Heart sample harvested at 12 weeks after ligation. LacZ+ cell (arrow) was morphologically integrated into the scar formation. (F) Heart sample harvested at 12 weeks after ligation, and immunostained for α-smooth muscle actin. LacZ+ cell (arrow) was seen in the vascular wall in the infarcted myocardium. (G) Heart sample harvested at 12 weeks after ligation, and immunostained for MF20. LacZ+ cells (arrows) had immunoreactivity for sarcomeric myosin heavy-chain molecules in their cytoplasms. (H) Heart sample harvested at 12 weeks after ligation, and immunostained for troponin I-C. LacZ+ cells (arrows) had immunoreactivity for troponin I-C in their cytoplasms. Original magnifications: (A) ×100; (B) ×200; (C through F) ×400; (G and H) ×1000.
The Annals of Thoracic Surgery  , 19-24DOI: ( /S (02) )