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Fig. 1 Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. Neutrophils.

Published byLucia Garofalo Modified over 5 years ago

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Presentation on theme: "Fig. 1 Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. Neutrophils."— Presentation transcript:

1 Fig. 1 Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. Neutrophils derived from patients with MPNs are associated with an increase in NET formation and a prothrombotic, NET-rich phenotype. (A) NET formation in patients with myeloproliferative neoplasms (MPNs) (receiving a JAK inhibitor, n = 5; receiving other therapy, n = 14) compared to healthy controls (n = 11) when stimulated with 4 μM ionomycin (IO) or dimethyl sulfoxide (DMSO) for 2 hours. Patients receiving a JAK inhibitor are indicated by JAKI. Data are shown as individual values and medians. (B) Representative IF images of human neutrophils after stimulation with 4 μM IO or DMSO for 2 hours. 4′,6-Diamidino-2-phenylindole (DAPI) is shown in blue, and citrullinated histone H3 (H3cit) is shown in green. Scale bar, 50 μm. (C) Percentages of human neutrophils with evidence of NET formation after stimulation with phorbol 12-myristate 13-acetate (PMA) (10 nM) with and without ruxolitinib pretreatment (n = 4). Neutrophils are derived from controls. (D) Representative images of human neutrophils from healthy controls stimulated with PMA (10 nM) after 150 min of ex vivo pretreatment with DMSO, ruxolitinib (300 nM), or GSK484 (PAD4 inhibitor,10 μM). Scale bar, 50 μm. (E) Lung tissue sections from mice expressing the Jak2V617F mutation as compared to Jak2WT mice. Scale bar, 200 μm. (F) Characterization of clot content in the lungs of Jak2V617 mice. Hematoxylin and eosin (H&E) stain. Scale bar, 50 μm. VWF, von Willebrand factor. (G) Lung tissue sections from mice expressing the Jak2V617F mutation as compared to Jak2WT mice. Neutrophil infiltration and NETs are shown by neutrophil-specific Ly6G (red) and H3cit (green), respectively. Scale bar, 100 μm. (H) Percentages of mouse neutrophils with evidence of NET formation by morphological criteria (left) (n = 9 for all genotype/treatment combinations) or H3cit-positive staining (right) (n = 6 for all genotype/treatment combinations) grouped by genotype after stimulation with 4 μM IO or DMSO for 2 hours. (I) Representative IF images of mouse neutrophils derived from Jak2WT and Jak2V617F mice after stimulation with 4 μM IO or DMSO for 2 hours. DAPI is shown in blue, and H3cit is shown in green. Scale bar, 50 μm. Ofir Wolach et al., Sci Transl Med 2018;10:eaan8292 Published by AAAS

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