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PD-1–PD-L1 axis is highly expressed and is not IFNγ-dependent in a subcutaneous murine model of PDA. A, experimental design for establishment of subcutaneous.

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Presentation on theme: "PD-1–PD-L1 axis is highly expressed and is not IFNγ-dependent in a subcutaneous murine model of PDA. A, experimental design for establishment of subcutaneous."— Presentation transcript:

1 PD-1–PD-L1 axis is highly expressed and is not IFNγ-dependent in a subcutaneous murine model of PDA. A, experimental design for establishment of subcutaneous PDA tumors or chronic LCMV clone 13 (Cl-13) infection simultaneously in two cohorts of C57BL/6 mice. PD-1–PD-L1 axis is highly expressed and is not IFNγ-dependent in a subcutaneous murine model of PDA. A, experimental design for establishment of subcutaneous PDA tumors or chronic LCMV clone 13 (Cl-13) infection simultaneously in two cohorts of C57BL/6 mice. B, representative flow plots and quantification of coexpression of PD-1 and Lag-3 on CD8+ (gated on live, lymphocytes, B220neg, NK1.1neg, CD8+), CD4+ (gated on live, lymphocytes, B220neg, NK1.1neg, CD4+) and regulatory (Tregs; gated on live, lymphocytes, B220neg, NK1.1neg, CD4+, FoxP3+) T cells from spleens of mice infected with LCMV Cl-13 (day 30) or the tumors and spleens of mice bearing PDA tumors (day 14). C, representative histograms and quantification of PD-L1 expression on tumor cells, DCs, and macrophages (Mac) in subcutaneous PDA tumors or spleens from the same mice (day 14), gated as in Fig. 1B. ****, P ≤ See also Supplementary Fig. S2. D, histogram of KPC-derived PDA cell line interrogated for PD-L1 expression in vitro with or without IFNγ in the culture, representative of three experiments. E, quantification and MFI of PD-L1 expression on tumor cells from subcutaneous PDA tumors established in either C57BL/6 (B6) or IFNγ−/− (IFNγ ko) mice with or without CD4+ and CD8+ T-cell depletion (TCD; day 16; n = 6-8 mice per cohort). F, quantification and MFI of PD-L1 expression on DCs and macrophages in subcutaneous PDA tumors grown in either B6 or IFN-γ ko mice with or without TCD (day 16; n = 6–8 mice per cohort). One-way ANOVA:%DCs PD-L1+, P = 0.015; DC PD-L1 MFI, P = ;%Macs PD-L1+, P = 0.58; Macs PD-L1 MFI, P = Post hoc test P values are indicated where statistically significant: *, P ≤ 0.05; **, P ≤ 0.01. Rafael Winograd et al. Cancer Immunol Res 2015;3: ©2015 by American Association for Cancer Research

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