1. The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2)‏
The transcriptional behavior of GREB1 changes with estrogen dose and exhibits considerable cell‐to‐cell variation (see also Fig EV2 and Movie EV2) ACell‐to‐cell variation in GREB1 expression at multiple E2 concentrations. Transcription was observed for 13 h in individual MCF7‐GREB1‐PP7 cells at different E2 concentrations. Trajectories of single cells are represented as color maps and are sorted from lowest (top) to highest (bottom) total RNA output (ΣRNA), calculated as area under the curve divided by the average signal from single transcripts (right). Color denotes the absolute number of nascent RNAs. The squared coefficient of variation (standard deviation2/mean2) of the total RNA output among the population is indicated (right). Dashed lines indicate exemplary cells shown in panels (B and C).BRepresentative time traces for low, medium, and high expressing cells.CAutocorrelation (ACF) curves of the individual time traces in (B).DIncreasing E2 increases the proportion and productivity of transcriptionally active periods. Histograms were generated from the number of RNA molecules at the transcription site from all data points at different E2 concentrations. The distribution of the background signal is shown in gray.E, FIncreasing E2 concentrations decrease the length of inactive periods and increase the burst size. Promoter OFF‐times (E) and burst sizes (F) were extracted from the experimental tracks (see Fig EV2E). Exponential functions (red) with the same mean (dashed line) are shown for the OFF‐times.
Christoph Fritzsch et al. Mol Syst Biol 2018;14:e7678
© as stated in the article, figure or figure legend