1. Volume 122, Issue 3, Pages 762-773 (March 2002)
CD40/CD154 ligation is required for the development of acute ileitis following oral infection with an intracellular pathogen in mice 
Wen Li, Dominique Buzoni–Gatel, Hajer Debbabi, Mark S. Hu, Franck J.D. Mennechet, Brigit G. Durell, Randolph J. Noelle, Lloyd H. Kasper 
Gastroenterology 
Volume 122, Issue 3, Pages (March 2002)
DOI: /gast
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2. Fig. 1
Histologic analysis. At day 7 after infection, (B) B6 mice showed major inflammation compared with (A) naive control. (C) CD154−/− mice and (D) anti-CD154–treated mice lacked evidence of intestinal inflammation at day 7 after oral infection. Chimeric mice were infected and killed at day 7 after infection. (E) CD40−/− → B6 Ly5.2 chimeric mice and (G) CD40−/− → CD40−/− chimeric mice showed only mild inflammation compared with (F) B6 → B6 Ly5.2 chimeric mice and (H) B6 → CD40−/− chimeric mice (bar = 100 μm).
Gastroenterology  , DOI: ( /gast )
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3. Fig. 2
Phenotypic analysis. A phenotypic analysis was performed on cells from 3 different intestinal compartments: lamina propria mononuclear cells (LPMC), Peyer's patches (PP), and mesenteric lymph nodes (MLN). This phenotypic analysis studied the association between different markers: CD40 and CD19 (B cells) or Mac-3 (macrophages) or CD11c (dendritic cells) into (1) B6 naive mice and B6 mice at days (2) 3 and (3) 7 after infection. This study also examined the expression of these markers at day 7 after infection in chimeric mice: (4) B6 → B6 Ly5.2 chimeric mice and (5) CD40−/− → B6 chimeric mice. Results are expressed as the percentage of CD40 positive (□) and among those positive for CD40 is expressed the percentage of double-positive cells for 3 different phenotypic markers (▩).
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

4. Fig. 3
CD154 expression. At day 3 after infection, there was evidence of CD154 expression in the (A) intestine (green, FITC), (B) Peyer's patches (red, PE), and (C) mesenteric lymph nodes (red, PE). At day 7 after infection, CD154 (red, PE) was coexpressed within CD4+ (green, FITC) T cells in (D) Peyer's patches and (E) mesenteric lymph nodes or within CD8α+ (green, FITC) T cells in (F) Peyer's patches.
Gastroenterology  , DOI: ( /gast )
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5. Fig. 4
Chemokines and cytokines production. RPA analysis for (A) chemokines and (B) cytokines was performed on the whole ileum from different groups of mice at day 7 after infection.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

6. Fig. 5
Systemic IL-12 production. Level of IL-12 (p70) into the sera from different groups was detected by enzyme-linked immunosorbent assay at increasing times after infection. Constitutive level of IL-12 p70 in sera of naive mice was 0.31 pg/mL.
Gastroenterology  , DOI: ( /gast )
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7. Fig. 6
Production of IL-12 at the gut level. Confocal analysis gives evidence of the overexpression of IL-12 (p40/p70) (red, PE) (A) into the intestine of B6 mice at day 3 after infection and (B) lower in the mesenteric lymph nodes. A higher expression of IL-12 was also detected in (C) B6 → B6 Ly5.2 chimeric mice in the intestine and (D) remained low in the mesenteric lymph nodes compared with the lower level in CD40−/− → B6 Ly5.2 chimeric group in the (E) intestine and (F) mesenteric lymph nodes. Bar = 50 μm.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions

8. Fig. 7
Parasite load. Parasite burden was evaluated by a polymerase chain reaction method in the spleen from the different experimental groups at day 7 after infection.
Gastroenterology  , DOI: ( /gast )
Copyright © 2002 American Gastroenterological Association Terms and Conditions