1. A B C D Supplementary Figure S1 UMSCC1 Plateau: 24 hr Pre-Treatment
24 hr Post-Treatment
Supplementary Figure S1. Survival of UMSCC1 cells as a function of treatment time with PF (10µM) treatment alone, a function of time with PF (10µM) treatment followed by irradiation (8 Gy), or a function of time with DMSO control (10µM) treatment followed by irradiation (8 Gy) (A). Survival as a function of PF (10µM) treatment time following irradiation (8 Gy) data not shown. Radiation survival curves for UMSCC1 plateau phase cells treated with PF (10µM, Dashed Curve) or with DMSO Control (10µM, Solid Curve) for 24 hours prior to irradiation (Pre-Treatment), (B), or 24 hours following irradiation (Post-Treatment) (C). Column chart demonstrating a single representative experiment of exponentially growing FaDu cells treated with PF (1µM) or with DMSO Control (1µM) for 24 hours prior to irradiation (Pre-Treatment) or 24 hours following irradiation (Post-Treatment) (D). Individual survival curves representative of n = 2-3 independent experiments fitted using the linear quadratic model. Dose modifying factors (DMFs) and drug treatment toxicities are shown in Table 1. D
FaDu Exponential Growth:
24 hr 1µM PF Treatment
Post-IR
Treatment
Pre-IR
Treatment