1. Peptide abundances are uniformly distributed across the capsid protein in the recoded T7 strain.
Peptide abundances are uniformly distributed across the capsid protein in the recoded T7 strain. Shown are the abundances of individual peptides within the capsid protein of the recoded strain, relative to the wild type. Data are from 9 min after infection. If translation consistently terminated before the stop codon in recoded gene 10, we would expect the relative peptide abundance to systematically vary with the location of the peptide. Therefore, we tested a model that includes an interaction between strain and peptide location, and a model that includes no interaction (see Materials and Methods). We found that a model in which an interaction is included between the strain and peptide location fits the peptide count data no better than a model without interaction ( respectively). Thus, in the recoded strain, there is no evidence of early translation termination.
Benjamin R. Jack et al. G3 2017;7:
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