1. Effect of Tenascin-C Deficiency on Chemically Induced Dermatitis in the Mouse 
Yoh-ichi Koyama, Masashi Kusubata, Atsushi Yoshiki, Noriko Hiraiwa, Tomoo Ohashi, Shinkichi Irie, Moriaki Kusakabe 
Journal of Investigative Dermatology 
Volume 111, Issue 6, Pages (December 1998)
DOI: /j x
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Identification of the tenascin-C gene by PCR analysis of genomic DNA. (a) Schematic representation of the structure of the targeting vector and the position of PCR primers: (i) targeting vector; (ii) endogenous allele; (iii) homologously mutated allele. S and AS denote sense primer and anti-sense primer, respectively. (b) PCR analysis of tenascin-C gene. By use of two pairs of primers, two bands of 208 bp and 391 bp were detected in +/+ mice, whereas only one band of 299 bp was found in –/– mice. In heterozygous +/– mice, all three bands were detected.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

3. Figure 2
Temporal changes of the ear thickness in dermatitis. Dermatitis was induced with (a) DNFB (mean ± SD, n = 12) and (b) TPA (n = 9) in wild-type BALB/CA mice and congenic TNKO mice. An asterisk indicates a significantly greater ear thickness in TNKO mice than in wild-type BALB/CA mice.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

4. Figure 3
Expression of tenascin-C in dermatitis. The expression of tenascin-C protein in DNFB-treated ear of wild-type BALB/CA mouse (a) and congenic TNKO mouse (b) was examined by western blot analysis. Application of DNFB to the ear of wild-type BALB/CA mice induced the expression of 230 kDa tenascin-C (upper arrowhead) in addition to the constitutively expressed 180 kDa isoform (lower arrowhead) on day 1 (lane 2), day 3 (lane 4), day 5 (lane 6), and day 7 (lane 8), whereas the vehicle alone did not (lane 1 for day 1,lane 3 for day 3,lane 5 for day 5, andlane 7 for day 7). In congenic TNKO mice, tenascin-C was never detected in the ear after vehicle application (lanes 1, 3, 5, 7) or DNFB application (lanes 2, 4, 6, 8) on day 1 (lanes 1, 2), day 3 (lanes 3, 4), day 5 (lanes 5, 6), or day 7 (lanes 7, 8). Asterisks indicate tissue-derived mouse immunoglobulins. Molecular weight markers are indicated on the left in kDa.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

5. Figure 4
Histology of the ear. Thin sections of the ear of wild-type BALB/CA mice (a) and TNKO mice (b, c) treated with DNFB (a, b) or TPA (c) were stained with hematoxylin and eosin. In the ear of wild-type BALB/CA mice treated with DNFB, infiltrating cells were mostly mononuclear cells on day 5, whereas many PMN [inset in (b) at a higher magnification] persisted in TNKO mice. Anarrow in (b) indicates the elastosis-like structure found in the ear of TNKO mice. In contrast to DNFB treatment, application of TPA induced the infiltration of PMN in the epidermis on day 1 (arrow) (c).Scale bars: 20 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions

6. Figure 5
Immunohistochemistry of the ear. Indirect immunofluorescence staining was performed on thin sections of the ear of wild-type BALB/CA mice (a, b, d, f) and TNKO mice (c, e, g, h), which were non-treated (a), treated with DNFB (b–e; day 5), or treated with TPA (g–h; day 1). In the ear of non-treated wild-type BALB/CA mice, tenascin-C was detected only in the subcutaneous tissue of the inner side (large arrow) and at the perichondrium (small arrow) (a). DNFB treatment induced the expression of tenascin-C in the outer side of the ear (b) as well as in the inner side; however, only nonspecific background staining was observed in the ear in congenic TNKO mice (c). A large number of Gr-1-positive PMN were retained in the ear on day 5 after DNFB treatment in TNKO mice (e), but only a few Gr-1-positive cells were found in wild-type mice (d). TPA treatment induced a massive infiltration of Gr-1-positive PMN on day 1 in both wild-type mice (f) and TNKO mice (g), and many of Gr-1-positive PMN were found in the epidermis (h).Arrowheads in (d–h) show Gr-1-positive PMN. T, cartilage.Scale bars: 20 μm.
Journal of Investigative Dermatology  , DOI: ( /j x)
Copyright © 1998 The Society for Investigative Dermatology, Inc Terms and Conditions