1. Mpdz was localized proximal to the apical surface of CPECs
Mpdz was localized proximal to the apical surface of CPECs AImmunofluorescence images of 10‐μm‐thick sections of CP from third ventricle villi of Mpdz+/+ and Mpdz−/− mice were immunolabeled as shown. The areas in the square frames are magnified in the bottom panels. Scale bars, top panels, 100 μm; bottom panels, 10 μm.B, CCP sections from lateral ventricle villi immunolabeled for ZO1 (B) or E‐cadherin (E‐cad; C). The images are representative of two Mpdz+/+ mice and three Mpdz−/− mice. Scale bars, top panels, 100 μm; bottom panels, 10 μm.DImmunoblots with the indicated antibodies (top) and their quantifications. The densitometry measurements were normalized relative to the signal of the samples transduced by non‐targeting shRNA (Ctrl). Note that the same β‐actin immunoblot was used twice because the Jam‐C and E‐cadherin samples were immunoblotted on the same membrane (mean ± SD, n = 3; the values of P were determined by two‐tailed Student's t‐test). Source data are available online for this figure.
Junning Yang et al. EMBO Mol Med. 2019;11:e9540
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